水产学报2017，Vol.41Issue(4)：602-612,11.DOI:10.11964/jfc.20160310314
添加外源益生菌对大菱鲆育苗生物饵料菌群结构的影响
The microflora changes of rotifer and artemia with probiotics addition in Scophthalmus maximus larva breeding
摘要
Abstract
Rotifer and artemia are indispensable living food in aquatic larval animal breeding.In order to evaluate bacterial communitiy change in rotifer and artemia enriching process with probiotics adding for turbot larva breeding,their microflora were analyzed using high-through sequencing technique based on illumina HiSeq platform.The results revealed that the diversity of experimental group with probiotics addition was higher than control group,which was the normal process in breeding.During rotifer enrichment process,the bacterial community structures of control group at different time were obviously different,and the dominant bacteria species varied greatly.However,bacterial community structures in different experimental groups were similar,and the dominant bacteria species were more diverse.Lactococcus sp.,Pseudoalteromonas sp.and Alteromonas sp.as main bacteria appeared in each experimental group.In artemia enrichment process,bacterial communities of each control group were highly similar.The dominant bacteria of Cobetia sp.relative abundance reached 54％-65.2％ in control group.With probiotic adding,bacterial community of each experimental group were still highly similar,but Cobetia sp.declined to 4.3％-25.3％,Pseudoalteromonas sp.and Alteromonas sp.became the new dominant bacteria.In conclusion,our findings showed that the bacterial community structure of living food for aquatic animal larva changed obviously and trended to be stable with probiotic adding,and the bacteria species in them became more uniform.关键词
大菱鲆/生物饵料/轮虫/卤虫/菌群结构/益生菌Key words
Scophthalmus maximus/living food/rotifer/artemia/probiotics/microbial community structure分类
农业科技引用本文复制引用
于永翔,姜燕,张正,王印庚,景亚运,廖梅杰,薛良义..添加外源益生菌对大菱鲆育苗生物饵料菌群结构的影响[J].水产学报,2017,41(4):602-612,11.基金项目
国家自然科学基金(31302206) （31302206）
山东省自主创新与成果转化专项(2014ZZCX06205) （2014ZZCX06205）
青岛市博士后人员应用研究项目 National Natural Science Foundation of China (31302206) （31302206）
Shandong Province Independent Innovation and Achievement Transformation Projects (2014ZZCX06205) （2014ZZCX06205）
Qingdao Postdoctoral Personnel Applied Research Projects （）
