山东医药2017，Vol.57Issue(18)：1-4,4.DOI:10.3969/j.issn.1002-266X.2017.18.001
胆固醇对人结肠癌细胞增殖、侵袭、迁移的影响及机制探讨
Effect of cholesterol on proliferation, invasion and migration of human colon cancer cells
摘要
Abstract
Objective To observe the effect of cholesterol on the proliferation, invasion and migration of human colon cancer cells and to discuss the possible mechanism.Methods The human colon cancer cells HT-29 in the logarithmic growth phase were divided into 6 groups: group A without any treatment, group B which was added with cholesterol for 48 h (final concentration: 1 μmol/L), group C/D and E/F which were transfected by negative siRNA-HiperFect complexes and Acyl coenzyme A cholesterol acyltransferase 1 (ACAT1) gene siRNA-HiperFect complexes using Lipofectamine 2000, respectively.After 48 h, the cells in the groups D and F received the same treatment of group B.CFSE staining assay was used to evaluate the proliferation of cells.Transwell assay was used to evaluate the migration and invasion of cells.The expression of ACAT1 gene was detected by real-time PCR.Results The cell proliferation rate, the cell invasion and migration numbers in the group B and D were higher than those of the other groups (all P<0.05), and no statistically significant difference was found between any two of groups A, C, E and F.The relative expression of ACAT1 mRNA in the groups A, B, C, D, E and F was 0.96±0.02, 1.48±0.05, 0.94±0.02, 1.44±0.02, 0.54±0.36 and 0.63±0.05, the relative expression of ACAT1 mRNA in the groups B and D was higher than that of the other groups (all P<0.05), the relative expression of ACAT1 mRNA in the groups E and F was lower than that of the other groups (all P<0.05), and no statistically significant difference was found between group A/C, B/D and E/F.Conclusion Cholesterol can improve the proliferation, migration and invasion of human colon cancer cells by promoting ACAT1 gene expression.关键词
结肠癌/胆固醇/胆固醇酰基转移酶1/细胞增殖/细胞侵袭/细胞迁移Key words
colon carcinoma/cholesterol/cholesterol acyltransferase 1/cell proliferation/cell invasion/cell migration分类
医药卫生引用本文复制引用
陈心,伍龙,张一桥,陈能,徐细明..胆固醇对人结肠癌细胞增殖、侵袭、迁移的影响及机制探讨[J].山东医药,2017,57(18):1-4,4.基金项目
国家自然科学基金资助项目(81302133) （81302133）
中央高校基本科研业务费专项基金资助项目(2042014kf0149) （2042014kf0149）
湖北省自然科学基金资助项目(2012FKC143). （2012FKC143）
