山东医药2017，Vol.57Issue(18)：23-25,3.DOI:10.3969/j.issn.1002-266X.2017.18.007
C型钠尿肽对肾缺血再灌注大鼠肾功能和肾组织细胞凋亡的影响及机制
Effects of CNP on renal function and renal apoptosis in rats with renal ischemia-reperfusion injury
摘要
Abstract
Objective To observe the effects of C-type natriuretic peptide (CNP) on renal function and renal tissue apoptosis in rats with renal ischemia-reperfusion (IR) injury and its mechanisms involved.Methods Twenty-four male Wistrar rats were randomly divided into the Sham group, IR group and CNP group (n=8).All the rats were left unilaterally nephrectomized.In the IR group and CNP group, we nipped the right renal artery and vein with noninvasive small vascular clip for 45 min.CNP [0.2 μg/(kg·min)] and 2 mL physiological saline was infused immediately and slowly at the start of a 45-min renal ischemia for 2 h in the two groups, respectively.The right renal artery and vein in the Sham group was not treated by the clip.Rats were killed to obtain the blood at 24 h after I/R.The serum creatinine (Cr) and urea nitrogen (BUN) was measured, cyclic guanosine monophosphate (cGMP) in right renal tissues was measured by ELISA, and the apoptotic cells in kidney tissues was detected by TUNEL staining.The renal tissue protein kinase B (Akt), β glycogen synthase kinase 3β (GSK-3β) protein and their phosphorylated protein was measured by Western blotting.Results Compared with the Sham group, the levels of serum Cr and BUN were significantly increased (both P<0.01), the concentration of cGMP in renal tissues was decreased, and the apoptosis rate was increased (both P<0.05), the protein phosphorylation levels of Akt and GSK-3β in the renal tissues were decreased in the IR group (both P<0.05).Compared with the IR group, the levels of serum Cr and BUN were decreased (both P<0.05), the concentration of cGMP was increased in the renal tissues and the apoptosis rate was decreased (both P<0.05), the protein phosphorylation levels of Akt and GSK-3β in renal tissues were increased in the CNP group (both P<0.01).Conclusions CNP can alleviate IR-induced kidney injury and renal tissue apoptosis.The mechanism may be related to the increased renal tissue cGMP levels which effectively increase Akt and GSK-3β protein phosphorylation.关键词
急性肾功能衰竭/缺血再灌注/C型钠尿肽/肾功能/细胞凋亡/蛋白激酶B/糖原合成酶激酶3β/大鼠Key words
acute kidney injury/ischemia-reperfusion/C-type natriuretic peptide/renal function/apoptosis/protein kinase B/glycogen synthase kinase-3β/rats分类
医药卫生引用本文复制引用
朴敏虎,金秀男,李香丹,崔逢德,许东元..C型钠尿肽对肾缺血再灌注大鼠肾功能和肾组织细胞凋亡的影响及机制[J].山东医药,2017,57(18):23-25,3.基金项目
国家自然科学基金资助项目(81460056). （81460056）
