上海农业学报2017，Vol.33Issue(2)：63-70,8.DOI:10.15955/j.issn1000-3924.2017.02.12
杜鹃根际微生物的分离、鉴定及多样性分析
Isolation, identification and diversity analysis of rhododendron rhizospheric microorganism
摘要
Abstract
In order to find out the effects of altitude and provenance on rhododendron rhizospheric colony composition,both rhizospheric and non-rhizospheric soils of the only two species of wild rhododendron in Beijing (Rhododendron mucronulatum and R.micranthum)were sampled at different altitudes,the bacterial and fungal purification and their molecular identification were made,and the soil DNAs were extracted and sequenced by a second-generation high-throughput sequencing.Also the impacts of different factors on the colony composition and abundance were analyzed according to the sampling sites' natural conditions.The results showed that isolating the rhizospheric soils obtained 11 species of bacteria under 5 genera and 9 species of fungi under 7 genera;The R.mucronulatum rhizospheric soils at low and high altitudes had 5 and 3 dominant genera respectively;The nonrhizospheric soils at low and high altitudes had 8 and 9 dominant genera respectively;The bacterial composition of rhododendron rhizospheric soils was more stable than that of non-rhizospheric soils and the dominant genera of rhizosphere had a certain directive property;The bacterial diversity of R.mucronulatum rhizosphere was greater than that of R.micranthum rhizosphere,but the dominant genera of R.mucronulatum rhizosphere were less than those of R.micranthum rhizosphere;Both bacterial diversity and dominant genera of R.mucronulatum rhizosphere at low altitude were greater than those at high altitude.It was concluded that both altitude and provenance significantly influenced the colony composition and abundance of rhododendron rhizosphere,and the bacterial diversity of rhododendron rhizosphere had a relationship with plant phenological phases and was different between different-provenance rhododendrons due to their different root exudates.Compared with the high-throughput sequencing result,the microbial species obtained by isolation was obviously less,which was identical with previous studies.关键词
杜鹃/根际微生物/鉴定/多样性Key words
Rhododendron/Rhizospheric microorganism/Identification/Diversity分类
农业科技引用本文复制引用
周田田,丁榕,蔡建超,赵和文,崔金腾..杜鹃根际微生物的分离、鉴定及多样性分析[J].上海农业学报,2017,33(2):63-70,8.基金项目
北京市科技提升计划(TJSHG201310020020) （TJSHG201310020020）
北京市属高等学校创新团队建设项目(IDHT20150503) （IDHT20150503）
