上海农业学报2017，Vol.33Issue(2)：85-88,4.DOI:10.15955/j.issn1000-3924.2017.02.16
人工合成高效启动子用于基因治疗及疫苗研制
Preparation of artificial synthetic promoter for gene therapy and vaccine development
摘要
Abstract
A promoter was constructed by this experiment,which could be used for the construction of gene therapy vector and vaccine development.According to the characteristics of the promoter,a sequence of specific promoter (SEP)was synthesized,which was inserted into pEGFP-C1 containing green fluorescent protein.The pEGFP-C1 plasmid containing a new promoter ESP was transfected into BHK-21 cells,the green fluorescent protein expression was observed by using a fluorescence microscopy.The results showed that the protein expression of pEGFP-ESP-C1 was significantly higher than that of pEGFP-C1,and the expression was stability.The results indicated that the promoter ESP could be used in the construction of gene therapy vector,and the expression level of the gene transferred into the cell was improved,which laid the foundation for the final success of gene therapy.关键词
启动子/基因治疗/疫苗研制Key words
Promoter/Gene therapy/Vaccine development分类
农业科技引用本文复制引用
刘成倩,李红,易建中,孙晓云..人工合成高效启动子用于基因治疗及疫苗研制[J].上海农业学报,2017,33(2):85-88,4.基金项目
上海市科学技术委员会重点科技攻关项目(12391901900) （12391901900）
