中国石油大学学报（自然科学版）2017，Vol.41Issue(2)：163-174,12.DOI:10.3969/j.issn.1673-5005.2017.02.020
3株原油降解细菌鉴定及其降解和驱油特性研究
Identification of 3 strains of crude oil degradation bacteria and its characteristics of degradation and oil-displacement
摘要
Abstract
The bacteria for microbial enhanced oil recovery (MEOR) were screened,and then the characteristics of degradation,fermentation and oil-displacement of the bacteria were investigated in this study.One crude oil as the sole carbon source was used to isolate the crude oil degrading strains by improved dilution-plate method.The bacteria strains with MEOR potential were screened through observing the change of bacteria colony growth in the plates and crude oil properties in fermentation flask.Then the strains were identified through the colony and cell morphology,physiological-biochemical tests and 16S rDNA sequence analysis.Also,the influences of strains on the chemical composition of crude oil were analyzed by using the gas chromatography and alumina column chromatography.Meanwhile,the oil displacement efficiency of the fermentation broth after bacteria fermentation was studied using the indoor simulation experiment.It is found that 3 strains with enhanced oil recovery potential of the 17 bacteria strains,which were isolated from the crude oil and oily soil,were screened for the follow-up tests,which were identified as Dietzia cerrcidiphylli (X10),Micrococcus yunnanensis (X19) and Pseudomonas aeruginosa (X22),respectively.The 3 strains of bacteria significantly change the chemical composition of crude oil.The asphaltene degradation rate of X19 reaches as high as 65.7％.The degradation of resin and aromatic hydrocarbon of X10 reaches 84.4％ and 32.15％,respectively.And the saturated hydrocarbon of X10,X19 and X22 is increased at different levels.It is also found that glycolipid surfactant could be produced by X22.The fermentation broth of X22 shows that its oil replacement efficiency is 33.5％ higher than water in the indoor simulation experiment,and the oil replacement efficiency is high in the low permeability or heterogeneous simulation medium.It is concluded that the 3 strains of bacteria have good potential of oil replacement,especially Pseudomonas aeruginosa with the excellent performance in the indoor simulation experiment,which is good for the study and application of MEOR.关键词
微生物强化采油/石油/细菌/生物表面活性物质Key words
microbial enhanced oil recovery (MEOR)/petroleum/bacteria/bio-surfactant分类
生物科学引用本文复制引用
马鑫,高卉,张俊会,王平,薛泉宏..3株原油降解细菌鉴定及其降解和驱油特性研究[J].中国石油大学学报（自然科学版）,2017,41(2):163-174,12.基金项目
国家科技支撑计划项目(2012BAD14B11) （2012BAD14B11）
陕西博秦生物工程有限公司微生物增油项目 （）
