天津科技大学学报2017，Vol.32Issue(2)：24-29,6.DOI:10.13364/j.issn.1672-6510.20160088
毕赤酵母热致死动力学模型的建立与检验
Construction and Verification of a Model for the Lethal Thermal Kinetics of Pichia pastoris
田康明 1段威 2王正祥 1路福平2
作者信息
- 1. 天津科技大学化工与材料学院,天津 300457
- 2. 工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津 300457
- 折叠
摘要
Abstract
Pichia pastoris,as one of the most mature protein overexpression platforms in the study of microorganisms,was widely used for bulk product manufacturing such as enzyme and functional macromolecule.Establishing a lethal thermal kinetics model of Pichiapastorisunder various heat treatment conditions from 50,℃ to 60,℃ will supply a guide to Pichia pastoris cell manufacturing system and increase the utilization value of cellmass.The lethal thermal kinetics of Pichiapas-torisunder various heat treatment conditions from 50,℃ to 60,℃ was investigated,and the availability and feasibility of the model was verified by live cell lethal tests.The cell culture of Pichiapastoris GS115 with different optical density was incu-bated at 50,℃,55,℃ and 60,℃ for various heating time respectively,then the obtained lethal curves were fitted usingthe Weibull model and calculated using multiple regression equation to establish the lethal thermal kinetics model.Subsequently the feasibility and pratical lethality were confirmed by live cell lethal tests.APichiapastoris lethal thermal kinetics model was thus established,and its availability and feasibility were confirmed.The established model in this study can be used to describe the lethal thermal kinetics of Pichiapastorisunder various heat treatment conditions.The methods constructed here can be used for getting the suitable heat treatment process for heat inactivation of Pichiapastoris.关键词
毕赤酵母/致死动力学/预测微生物学/Weibull模型Key words
Pichia pastoris/death kinetics/predictive microbiology/Weibull model分类
生物科学引用本文复制引用
田康明,段威,王正祥,路福平..毕赤酵母热致死动力学模型的建立与检验[J].天津科技大学学报,2017,32(2):24-29,6.
