厦门大学学报（自然科学版）2017，Vol.56Issue(3)：331-338,8.DOI:10.6043/j.issn.0438-0479.201611033
白骨壤重金属耐受性相关基因AmNramp3的克隆与分析
Cloning and Analysis of Heavy Metal Tolerance-associated Gene AmNramp3 in Avicennia marina (Forsk.) Vierh.
摘要
Abstract
The halophytic Avicennia marina (Forsk.) Vierh.is one of the pioneer mangroves along the southern coast of China,which is a good material for understanding molecular mechanisms behind heavy metal pollutant tolerance in mangrove plants.In this study,based on the result of suppressive subtractive hybridization using rapid amplification of cDNA ends (RACE) technology,AmNramp3 gene was cloned using gene specific primers,with the full-length cDNA of 1 958 bp.We analyzed the expression of the AmNramp3 gene in leaf,stem and root after Cd treatment based on the quantative real-time PCR (qRT-PCR),and found the expression level in leaf was higher than in stem and root,while exhibited rapid response in stem and root.Additionally,the open reading frame (ORF) of the AmNramp3 gene was predicted and analyzed using bioinformatics,and results show that it is located in 1 bp to 1 527 bp and encodes 508 amino acids.Then the structure of the AmNramp3 protein was predicted and analyzed,and results show that the protein has no signal peptide,indicating that it is not a secretory protein.However,the AmNramp3 protein has a complex transmembrane structure,indicating that it is a transmembrane protein.Therefore,our analysis predicts that AmNramp3 encodes a transmembrane protein and the expression is rapidly changed under Cd stress,which is possibly related to the transmembrane transport function of heavy metal cadmium.This is helpful for us to understand the molecular mechanism of the AmNramp3 gene function in terms of heavy metal tolerance.关键词
白骨壤/重金属耐受性/镉/NrampKey words
Avicennia marina/heavy metal tolerance/Cd/Nramp分类
农业科技引用本文复制引用
张骥诚,梁嘉庆,俞锦锋,刘景春,卢豪良,严重玲..白骨壤重金属耐受性相关基因AmNramp3的克隆与分析[J].厦门大学学报（自然科学版）,2017,56(3):331-338,8.基金项目
国家自然科学基金(31530008,31370516) （31530008,31370516）
