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黏度法研究牛血清白蛋白的构象

廖芸英 熊晓鹏

厦门大学学报(自然科学版)2017,Vol.56Issue(3):456-460,5.
厦门大学学报(自然科学版)2017,Vol.56Issue(3):456-460,5.DOI:10.6043/j.issn.0438-0479.201606036

黏度法研究牛血清白蛋白的构象

Conformation of Bovine Serum Albumin Studied with Viscometric Method

廖芸英 1熊晓鹏1

作者信息

  • 1. 厦门大学材料学院,福建厦门361005
  • 折叠

摘要

Abstract

The viscosities of bovine serum albuminin (BSA) in different pH buffers were measured using Ubbelohde capillary viscometer,and the data were evaluated by Wolf equation to describe the composition dependences of BSA.The intrinsic viscosities ([η]) of BSA under different pH were correlated with its conformation,and the composition dependences of specific intrinsic viscosities ({ η}) of BSA were carefully analyzed.Our results indicate that the new viscometric method can be applied in the study of conformation and solution properties of spherical protein.

关键词

黏度法/特性黏数/牛血清白蛋白/构象

Key words

viscometric method/intrinsic viscosity/bovine serum albumin(BSA)/conformation

分类

化学化工

引用本文复制引用

廖芸英,熊晓鹏..黏度法研究牛血清白蛋白的构象[J].厦门大学学报(自然科学版),2017,56(3):456-460,5.

基金项目

国家自然科学基金(51273166) (51273166)

厦门大学学报(自然科学版)

OA北大核心CSCDCSTPCD

0438-0479

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