新医学2017，Vol.48Issue(5)：302-307,6.DOI:10.3969/j.issn.0253-9802.2017.05.004
甲型流感病毒H1N1/FM1对小鼠巨噬细胞NLRP3炎症小体信号通路的影响
Effect of influenza A virus H1N1/FM1 on macrophage NLRP3 inflammosome signaling pathway in mouse
摘要
Abstract
Objective To investigate the variation of NOD-like receptor P3 (NLRP3) inflammosome signaling pathway at different time points in mouse macrophages infected by influenza A virus H1N1/FM1. Methods Mouse monocyte-macrophage cell line RAW264.7 was used in this experiment. In the study group, the cells were infected with H1N1/FM1 for 1 week, and those were infected for 6 h in the control group. The expression of IL-1β in the cell supernatant was measured by ELISA. The relative expression of NLRP3, caspase-1 and IL-1β mRNA was investigated by fluorescent quantitative PCR. The expression levels of NLRP3, caspase-1 and cleaved caspase-1 proteins were quantitatively detected by Western blot. The expression of NLRP3 protein was observed by immunofluorescent staining. Results In the study group, the expression of IL-1β in the cell supernatant was significantly up-regulated (P<0.01), the expression levels of NLRP3 mRNA and protein were significantly down-regulated (both P<0.05), the relative expression of caspase-1 and IL-β mRNA, and caspase-1 and cleaved-caspase-1 proteins were significantly up-regulated (all P<0.05) compared with those in the control group. The fluorescent intensity of NLRP3 protein in the study group was lower than that in the control group. Conclusions Infection of macrophage cells with influenza A virus H1N1/FM1 can activate the NLRP3 inflammosome signaling pathway and promote the secretion of IL-1β. Futhermore, the activating effect can be enhanced during the late stage of infection (approximately 1 week).关键词
甲型流感病毒H1N1/FM1/NOD样受体P3炎症小体/白介素-1βKey words
Influenza A virus H1N1/FM1/NOD-like receptor P3 inflammosome/Interleukin-1β引用本文复制引用
师小函,吴本权,石云锋,杨洋,朱家馨,张天托..甲型流感病毒H1N1/FM1对小鼠巨噬细胞NLRP3炎症小体信号通路的影响[J].新医学,2017,48(5):302-307,6.基金项目
国家自然科学基金(81170004) （81170004）
