疑难病杂志2017，Vol.16Issue(6)：614-617,4.DOI:10.3969/j.issn.1671-6450.2017.06.018
神经调节蛋白-1对心肌梗死大鼠心脏交感神经重构的保护效应研究
Effect of neuregulin-1 on cardiac sympathetic neural remodeling in rats with myocardial infarction
摘要
Abstract
Objective To investigate the effect of neuregulin 1 (NRG-1) on cardiac sympathetic neural remodeling in rats with myocardial infarction (MI).Methods The experiment was carried out in the Institute of cardiovascular disease, Wuhan University from September 2014 to December 2014.SD rats were randomly divided into sham operation group (SO group,n=6), myocardial infarction group (MI group,n=6) and NRG-1 group (n=6).Rats in MI and NRG-1 group underwent coronary artery ligation.NRG-1 (0.01 μg/g) was injected through the tail vein for seven days after MI operation in NRG-1 group.The same volume of saline was given to the rats in MI group.Rats in the SO group underwent thoracotomy and pericardiotomy but without ligation.Seven days after the operation, the Real Time PCR and Western blot technology were used to detect the mRNA and protein amount of TH (tyrosine hydroxylase, TH) and GAP43 (growth associated protein 43, GAP43) in the myocardial tissues from the infarct border zone.Results Compared with the SO group, the level of TH and GAP43 mRNA in the infarct border zone in the MI group increased significantly, (1.56±0.07 vs.0.76±0.27,P=0.001;1.32±0.09 vs 0.98±0.13,P=0.004), the administration of NRG-1 for 7 days decreased the mRNA level of TH (0.71±0.10) and GAP43 (0.96±0.06) in the infarct border zone of the NRG 1 group, which were significant lower than those of the MI group (P=0.001,P=0.018).The changes of the protein expression of TH and GAP43 were consistent with the changes of mRNA level in the three groups as the increased protein expression of TH and GAP43 in the MI group and the decreased expression in the NRG 1 group.Conclusion NRG-1 showed elimination effects in sympathetic neural remodeling after myocardial infarction, which is beneficial to improve cardiac electrical stability and contractile function.关键词
神经调节蛋白-1/心肌梗死/交感神经重构/大鼠Key words
Neuregulin-1/Myocardial infarction/Sympathetic neural remodeling/Rats引用本文复制引用
孔荆荆,来欣,魏雅念,沈成,王龙..神经调节蛋白-1对心肌梗死大鼠心脏交感神经重构的保护效应研究[J].疑难病杂志,2017,16(6):614-617,4.基金项目
湖北省自然科学基金资助项目(2014CKB497) （2014CKB497）
湖北省科技支撑计划项目(2014BKB075,2015BKA339) （2014BKB075,2015BKA339）
