云南农业大学学报（自然科学）2017，Vol.32Issue(2)：204-211,8.DOI:10.16211/j.issn.1004-390X(n).2017.02.003
云南省粘虫的遗传多样性
Genetic Diversity of Mythimna separata (Walker)(Lepidoptera: Noctuidae) in Yunnan Province
摘要
Abstract
The oriental armyworm,Mythimna separata (Walker) is a major agricultural and migratory pest in Yunnan Province.To verify the genetic diversity of oriental armyworm,the genetic diversity and genetic structure of 23 M.separata populations in Yunnan Province were analyzed by inter-simple sequence repeat (ISSR).The results showed that 9 primers were selected among 100 primers and 198loci were detected by ISSR marker analysis.The percentage of polymorphic loci (P) was 44.95％-66.16％,the Xisa population was the highest among 23 populations,the lowest polymorphic loci was in the Midu population.Coefficient of gene differentiation (Gst) among 23 populations was 0.3,showed that the genetic variation among individuals in the same population (70％) was higher than that of among populations (30％).Gene flow (Nm) among those populations was 1.669,the average of genetic similarity was 0.898 2,and the Mantel test results suggested that there was no significant correlation between the genetic distance and geographic distance of different M.separata populations (r =-0.0856,P =0.2820).Overall,the genetic diversity of armyworm in Yunnan Province was high,and there was genetic differentiation among different geographic populations.Moreover,the population genetic structure similarity was high and armyworm could prevent genetic drift caused by genetic differentiation among those 23 populations.The results in this paper can provide some new ideas for integrated pest management of M.separata.关键词
粘虫/遗传多样性/遗传分化/ISSRKey words
Mythimna separate/genetic diversity/genetic differentiation/ISSR分类
农业科技引用本文复制引用
陈晓燕,李亚红,郝若诗,可芮,李永川,赵世平,桂富荣..云南省粘虫的遗传多样性[J].云南农业大学学报（自然科学）,2017,32(2):204-211,8.基金项目
国家自然科学基金项目(31260450,30860069) （31260450,30860069）
云南省科技人才培养计划项目(2013HB072) （2013HB072）
云南省植保植检站项目. （）
