云南农业大学学报（自然科学）2017，Vol.32Issue(2)：218-225,8.DOI:10.16211/j.issn.1004-390X(n).2017.02.005
云南不同地区蜘蛛兰上朱顶红褪绿环斑病毒RNA S序列分析
S RNA Analysis of Hippeastrum Chlorotic Ringspot Virus on Spider Lily from Different Areas in Yunnan Province
摘要
Abstract
Hippeastrum chlorotic ringspot virus (HCRV) is a new Tospovirus species which was first discovered and reported in 2013 in Yunnan Province.HCRV induced concentric ring lines,leaf malformation,and necrotic spots symptoms on spider lily.Six samples infecting HCRV were selected by RT-PCR from different regions of Yunnan Province in this study.Six samples of the RNA S sequences were obtained through cloning,transformation and sequencing.The length of S sequence of the six isolates ranged from 2 714 to 2 747 nt,and the coding gene NSs coding the nonstructural protein by the S RNA (NSs) is 1 332 nt,N gene coding the nucleocapsid protein (N) is 825 nt.The relationships among different HCRV strains were determined by bioinformatics analysis.And we did mutation analysis and recombination detection of RNA S sequence and the protein coding.The results showed that mutation and recombination events were detected in all S RNA sequences of the six HCRV isolates.Combined with the environmental information collected from six separate objects,it was seen that there were great difference between the climate type,temperature and other environmental information in six collection cites,which suggested that the various climate types of Yunnan might relate to HCRV's wide distribution in Yunnan.Rapid mutation and recombination in these six HCRV isolates may be one of the reasons for the rapid adaptation of HCRV to different environments and a wide range of distribution.Finally,through the systematic evolution analysis,it is inferred that the HLS-TCRH collected from Baoshan,Yunnan was the first to evolve in the six isolates,it is divided into two clades,one branch includes YN-YX-HT-1 in Yuxi and HLS-YNRL-1 in Dehong Ruili,the other branch includes HLS-YL in Kunming,HLS-HHXY in Honghe and HLS-LC-YLHW in Lincang.关键词
蜘蛛兰/番茄斑萎病毒属/朱顶红褪绿环斑病毒/突变/重组Key words
spider lily/Tospovirus/Hippeastrum chlorotic ringspot virus/mutation/recombination分类
农业科技引用本文复制引用
高君直,吴保为,高雪,白继龙,李文贵,飞进强,刘雅婷,李永忠..云南不同地区蜘蛛兰上朱顶红褪绿环斑病毒RNA S序列分析[J].云南农业大学学报（自然科学）,2017,32(2):218-225,8.基金项目
国家自然科学基金项目(31260451,31471828) （31260451,31471828）
云南省重点新产品开发计划项目(2014BB005) （2014BB005）
国家级大学生创新项目(201510676002) （201510676002）
云南省大学生创新项目(2016106760015) （2016106760015）
云南农业大学研究生创新项目(2015ykc16). （2015ykc16）
