云南农业大学学报（自然科学）2017，Vol.32Issue(2)：358-365,8.DOI:10.16211/j.issn.1004-390X(n).2017.02.023
LC-MS技术快速识别迷迭香中黄酮苷类化合物
Fast Recognized the Flavoniod Glycosides from Rosmarinus Officinalis Linn by LC-MS Technique
摘要
Abstract
To determine the chemical component of Rosmarinus officinalis and study the fragmentation rules of the main constituent,the crude extract of R.officinalis Linn was analyzed by LC-MS technique.Seventeen compounds including one oligosaccharide,two phenylpropanoids and fourteen flavonoid glycosides were determined by LC-MS combined with Shimadzu Composition Formula Predictor and database searching.The main fragmentation pathway of flavonoid glycosides was losing the saccharide groups in MSn analyses,such as glucose (-m/z 162),rhamnose (-m/z 146),glucuronic acid (-m/z 176),acetyl glucuronic acid (-m/z 218) and rutinose (-m/z 308).RetroDiels-Alder reaction (RDA) scission was observed for some compounds which was useful for identifying the substituent groups on the flavonoid skeletons.It is suggested that LC-MS as an useful technique for quality analyzing in plant materials is fit for indentifying the flavonoid glycosides of R.officinalis.关键词
液质联用/裂解途径/迷迭香/黄酮苷Key words
LC-MS/fragmentation pathways/Rosmarinus officinalis Linn/flavonoid glycosides分类
化学化工引用本文复制引用
党立志,张翼鹏,张健,陶鹰,李向珍,段焰青..LC-MS技术快速识别迷迭香中黄酮苷类化合物[J].云南农业大学学报（自然科学）,2017,32(2):358-365,8.基金项目
云南省科学技术厅重点新产品开发计划项目(2012BA015) （2012BA015）
云南省科学技术厅引导类科技计划项目([2014] 01号) （[2014] 01号）
中国烟草总公司科技重点项目(110201402040) （110201402040）
云南省技术创新人才项目(2016HB009). （2016HB009）
