中国病理生理杂志2017，Vol.33Issue(5)：782-787,6.DOI:10.3969/j.issn.1000-4718.2017.05.003
ELMO1在胃癌细胞侵袭和迁移中的作用
Role of ELMO1 in invasion and migration of gastric cancer cells
摘要
Abstract
AIM:To reveal the role and function of engulfment and cell mobility 1 (ELMO1) in the invasion and migration of gastric cancer cells.METHODS:The expression of ELMO1 at protein and mRNA levels was detected in 5 kinds of gastric cancer cells and 1 normal human gastric epithelial cells by Western blot and real-time PCR, and the gastric cancer cells with the highest expression of ELMO1 were screened out.The cell transfection experiment was used to silence ELMO1 expression in the gastric cancer cells, and the effect of ELMO1 silencing on the invasion and migration of the gastric cancer cells was detected by Transwell assay.RESULTS:The expression level of ELMO1 in the gastric cancer cells was significantly higher than that in human normal gastric epithelial cells (P<0.01), and the SGC7901 cells had the highest expression level of ELMO1.ELMO1-siRNA significantly silenced the expression of ELMO1 in the SGC7901 cells (P<0.01).Silencing of ELMO1 expression significantly reduced the invasion and migration abilities of the human gastric can-cer cells (P<0.01).CONCLUSION:ELMO1 is highly expressed in the gastric cancer cells, and promotes the invasion and migration abilities of the gastric cancer cells.ELMO1 may become an effective target for the treatment of invasion and metastasis of gastric cancer.关键词
吞噬和细胞运动蛋白1/胃癌/细胞侵袭/细胞迁移Key words
Engulfment and cell mobility 1/Gastric cancer/Cell invasion/Cell migration分类
医药卫生引用本文复制引用
郭称明,彭会云,高典,胡雁,余琼芳,罗文..ELMO1在胃癌细胞侵袭和迁移中的作用[J].中国病理生理杂志,2017,33(5):782-787,6.基金项目
国家自然科学基金资助项目(No.81460462) （No.81460462）
南昌大学研究生创新基金资助项目(No.cx2015170) （No.cx2015170）
