中国康复理论与实践2017，Vol.23Issue(5)：514-519,6.DOI:10.3969/j.issn.1006-9771.2017.05.005
脂多糖对大鼠星形胶质细胞Ski蛋白表达的影响
Effect of Lipopolysaccharide on Ski Expression in Rats' Astrocytes
摘要
Abstract
Objective To explore how and where ski expresses under lipopolysaccharide (LPS) in rats' astrocytes. Methods Astrocytes were obtained from cerebral cortex of a newborn (within 3 days) Sprague-Dawley rat and cultured in vitro. Astrocytes were cultured with LPS in concentration of 0μg/ml, 0.001μg/ml, 0.01μg/ml, 0.1μg/ml, 1μg/ml, 10μg/ml and 100μg/ml for six hours;and cultured with LPS in concentration of 0.1μg/ml for 0 day, 2 days, 4 days, 6 days and 8 days. The level of ski was determined with Western blotting, and the lo-cation of ski was detected with indirect immunofluorescent staining. Results The expression of ski was induced by LPS, especially in the concentration of 0.1μg/ml. The expression of ski induced with 0.1μg/ml LPS peaked at 4 days of inducement and then decreased. Ski was mainly observed in nuclear in the normal astrocytes and the astrocytes induced with 0.1μg/ml LPS for 6 days. However, it was observed in cytoplasm 2 and 4 days of inducement. Conclusion LPS could induce the expression of ski in rats' astrocytes, which may participate in in-flammation.关键词
ski蛋白/脂多糖/炎症/星形胶质细胞/大鼠Key words
ski/lipopolysaccharide/inflammation/astrocyte/rats分类
医药卫生引用本文复制引用
赵鑫,郭永强,王明,陈铁戈,伍亚民,张海鸿..脂多糖对大鼠星形胶质细胞Ski蛋白表达的影响[J].中国康复理论与实践,2017,23(5):514-519,6.基金项目
国家自然科学基金项目(No.30772299). （No.30772299）
