中国临床药理学与治疗学2017，Vol.22Issue(3)：241-247,7.
脂多糖诱导的慢性帕金森病小鼠模型α-突触核蛋白的出现和转移变化
LPS-induced chronic Parkinson's disease in mice α-synuclein in the emergence and metastasis
摘要
Abstract
AIM:To establish a mouse model of chronic Parkinson's disease(PD) induced by lipopolysaccharide (LPS) and to investigate the behavioral changes of PD mice and the appearance and metastasis of α-synuclein(α-Syn) aggregates in the intestinal wall.METHODS:48 male C57BL/6J mice were randomly divided into normal saline control group and PD model group at 6 weeks of age.The PD mice model was established by intraperitoneal injection of LPS.Behavioral changes of the experimental mice were detected by the rotating bar test and field test.The dopaminergic neuron loss and α-Syn were observed by immunohistochemistry nuclear protein expression and metastasis.RESULTS:Behavioral changes of PD model mice exhibited significance difference (P ＜ 0.01).PD model mice's substantia nigra pars compacta α-Syn aggregation first appeared in the gut,and over time,the α-Syn was transferred to the substantia nigra of the mesencephalon,and the dopaminergic neurons were severely damaged.CONCLUSION:In the LPS-induced PD model,the typical pathological changes (α-Syn aggregation) begin in the colon nerves,and gradually develop into the substantia nigra pars compacta,resulting in dopaminergic neuron damage and loss.关键词
脂多糖/帕金森病/α-突触核蛋白/多巴胺能神经元Key words
lipopolysaccharide/Parkinson's disease/α-synuclein/dopaminergic neurons分类
医药卫生引用本文复制引用
葛波波,屈洪党,陈育华,胡彩彩,李理,姜牧君,许娟娟..脂多糖诱导的慢性帕金森病小鼠模型α-突触核蛋白的出现和转移变化[J].中国临床药理学与治疗学,2017,22(3):241-247,7.基金项目
国家自然科学基金(81641050) （81641050）
安徽省教育厅重点项目(KJ2104A163) （KJ2104A163）
蚌埠医学院研究生创新计划(Byycx1519) （Byycx1519）
