中国临床药理学与治疗学2017，Vol.22Issue(3)：248-252,5.
艾叶挥发油致小鼠急性肝毒性作用及其机制研究
Effect and mechanism of acute liver injury induced by volatile oil from folium artemislae argyi in mice
摘要
Abstract
AIM:To explore the effect and mechanism of acute liver injury induced by volatile oils from artemislae argyi folium in mice.METHODS:Volatile oil from folium artemislae argyi was obtained through hydro-distillation extraction.52 SPF ICR mice (half male and half female) were randomized into five groups,i,e.control group,carbon tetrachloride group,different doses (1.9 g/kg,2.3 g/ kg and 2.7 g/kg) of volatile oils from folium artemislae argyi groups.Biological samples were obtained 6 hours after gavaging equal volume drugs or solvents to mice at a single dose.Alanine amino transferase (ALT),alkaline phosphatase (ALP) and total bilirubin (TB) in serum were detected.Morphologic changes of hepatic tissues were observed and analyzed by Ridit.Superoxide anion free radical,superoxide dismutase (SOD),malondialdehyde (MDA),reduced glutathione (GSH) and oxidized glutathione (GSSG) of hepatic tissues were examined.RESULTS:Compared to those of control group,ALT,ALP,MDA,GSSG,scores of Ridit and superoxide anion free radical of all volatile oil groups increased (P ＜ 0.05),while SOD and GSH decreased (P ＜ 0.05).Damage of hepatic tissues,such as degeneration,necrosis were observed in the poisoned groups.CONCLUSION:The acute liver injury induced by volatile oil from folium artemislae argyi might directly or indirectly relate to hepatic cells,and the mechanism may be related to oxidative stress.关键词
艾叶挥发油/小鼠/急性肝毒性/氧化应激Key words
volatile oils from folium artemislae argyi/mice/acute liver injury/oxidative stress分类
医药卫生引用本文复制引用
刘红杰,李天昊,詹莎,陈亮,陈灵修,唐树杰..艾叶挥发油致小鼠急性肝毒性作用及其机制研究[J].中国临床药理学与治疗学,2017,22(3):248-252,5.基金项目
国家自然科学基金青年科学基金项目(30901936) （30901936）
广东省中医药局科研项目(20151185) （20151185）
