中国临床药理学与治疗学2017，Vol.22Issue(3)：253-259,7.
独一味乙醇提取物诱导人黏液表皮样癌MEC-1细胞凋亡的机制研究
Mechanism of apoptosis of mucoepidermoid carcinoma MEC-1 cells induced by ethanol extract of Lamiophlomis rotate
摘要
Abstract
AIM:To investigate the pro-apoptotic of ethanol extract of Lamiophlomis rotate (EELR) on human mucoepidermoid cancer cells (MEC1).METHODS:Sulforhodamine B (SRB) colorimetric method,cell clone formation experiment,Hoechst33258/PI staining and flow cytometry instrument technique were used to determine cell apoptosis,and Western blot analysis was used to detect alterations in cell apoptosis pathway-related proteins.RESULTS:EELR dose-and time-dependently exhibited anti-proliferation.With the increase of EELR concentration,the pro-apoptotic effect on MEC-1 was increased gradually.Moreover,Western blot analysis indicated that the expression of Bcl-2 was reducedand Bax and Casepase-3 were increased with the increasing of EELR concentration.CONCLUSION:EELR can induce MEC-1 cells apoptosis,which may be associated with the down-regulation of the Bcl-2 expression and up-regulation of the Bax and Casepase-3 expressions.关键词
独一味/人黏液表皮样癌MEC-1细胞株/细胞凋亡Key words
ethanol extract of Lamiophlomis rotate/mucoepidermoid carcinoma MEC-1 cell lines/cell apoptosis分类
医药卫生引用本文复制引用
马成华,周海静,施文,王志强,杨兰,陈萍,郑雪莉,马瑞,马丽娟..独一味乙醇提取物诱导人黏液表皮样癌MEC-1细胞凋亡的机制研究[J].中国临床药理学与治疗学,2017,22(3):253-259,7.基金项目
甘肃省科技支撑计划项目(1504FKCA091) （1504FKCA091）
西北民族大学中央高校基本科研业务费专项资金项目(31920130029) （31920130029）
国家民委科研项目(12XBZ012) （12XBZ012）
