中国临床药理学与治疗学2017，Vol.22Issue(3)：267-271,5.
地佐辛复合酮咯酸氨丁三醇超前镇痛对大鼠术后痛阈和炎性反应的影响
Effect of preemptive analgesia with dezocine + ketorolac tromethamine complex on postoperative pain thresholds and inflammatory reaction in rats
摘要
Abstract
AIM:To explore the effects of preemptive analgesia with dezocine + ketorolac tromethamine complex on postoperative pain thresholds and inflammatory reactions in rats.METHODS:A total of 60 adult Sprague Dawley rats were randomized into the control group,the preemptive analgesia (CZ) group and the postoperative analgesia (SZ) group,each with 20 rats.The rats were subjected to an abdominal midline incision followed by sterile suturing.In each group,5 rats were taken,and the pain thresholds were determined at 0,6,12 or 24 h after operation,respectively,using BL-420 biological and functional experimental system.Then,blood samples were collected from the inferior vena.After isolation,the expression of tumor necrosis factor-α (TNF-α) and interleukin1β (IL-1β) in serum were determined using enzyme-linked immunosorbent assays.The expression of substance P (SP) were observed using western blot analysis.RESULTS:There was no significant difference in the pain threshold,the levels of TNFα,IL-1β and SP of the three groups at 0 h (P ＞ 0.05).But at 6,12 or 24 h,the pain threshold of N group was significantly lower than that of CZ and SZ group (P ＜ 0.01).The levels of TNF-α and IL-1β of N group were significantly higher than those of CZ and SZ group (P ＜ 0.01) at 6,12 or 24 h,and although the levels of the CZ group and the SZ group did not show significant difference at 6 h (P ＞ 0.05),the levels of SZ group were significantly higher than the CZ group at 12 and 24 h (P ＜ 0.01).The levels of SP of N group were significantly higher than those of CZ and SZ group (P ＜0.01) at 6,12 or 24 h,and although the levels of the CZ group and the SZ group did not show significant difference at 6 and 12 h (P ＞ 0.05),the levels of SZ group were significantly higher than the CZ group at 24 h (P ＜ 0.01).CONCLUSION:Dezocine + ketorolac tromethamine inhibited the expression of the inflammatory factor TNF-α,IL-1β,and the pain factor SP after operation,showing a exact analgesic effect.Compared with postoperative application,the preemptive had a better effect.关键词
地佐辛/酮咯酸氨丁三醇/超前镇痛/炎性因子/P物质Key words
dezocine/ketorolac tromethamine/preemptive analgesia/inflammatory factor/substance P分类
医药卫生引用本文复制引用
常永丽,许银红,温雪敏,郭桢楠,张丽慧,韩颖,郭晓姝..地佐辛复合酮咯酸氨丁三醇超前镇痛对大鼠术后痛阈和炎性反应的影响[J].中国临床药理学与治疗学,2017,22(3):267-271,5.基金项目
创伤、烧伤与复合伤国家重点实验室开放课题(SKLKF201321) （SKLKF201321）
山西省大学生创新创业训练计划项目(2016312) （2016312）
