中国农业科技导报2017，Vol.19Issue(5)：22-27,6.DOI:10.13304/j.nykjdb.2016.351
转苋色藜NDR1基因水稻的获得及对白叶枯病抗性的初步研究
Transgenic Rice with Chenopodium amaranticolor NDR1 Genes and Preliminary Investigation on Bacterial Blight Resistance
摘要
Abstract
NDR1 (non-race-specific disease resistance 1) gene can mediate broad-spectrum in plant disease resistance and plays an important role in defense responses.Two NDR1 homologous genes CaNDR1a and CaNDR1b were cloned from Chenopodium amaranticolor,and plant expression vectors were constructed and generated transgenic rice by agrobacteria-mediated transformation,respectively.PCR and Southern blot assay demonstrated that those two genes were integrated into rice genome.Parts of transgenic lines were selected to conduct identification of resistance to rice bacterial leaf blight.The length of leaf disease spot were recorded in 3 d,5 d,10 d after inoculation.Results indicated that the expression of CaNDR1a and CaNDR1b in rice had certain resistance to bacterial leaf blight and delayed symptom development of the pathogen.Expression levels of exogenous genes in transgenic rice plants were significantly higher than those of control.However,higher disease resistance line did not show the highest expression results of CaNDR1.There were not significant different with receptor rice in agronomic traits such as seed setting rate and thousand seed weight,besides of shorter plant.关键词
NDR1/转基因水稻/白叶枯病/抗性鉴定Key words
NDR1/transgenic rice/bacterial leaf blight/disease resistance分类
生物科学引用本文复制引用
战园,张超,占锦,张永强..转苋色藜NDR1基因水稻的获得及对白叶枯病抗性的初步研究[J].中国农业科技导报,2017,19(5):22-27,6.基金项目
国家转基因生物新品种培育重大专项(2014ZX08001-002)资助. （2014ZX08001-002）
