中国农业科技导报2017，Vol.19Issue(5)：51-59,9.DOI:10.13304/j.nykjdb.2016.718
一株咔唑降解菌的鉴定及其降解特性研究
Identification and Characterization of a Carbazole-Degrading Bacterial Strain
摘要
Abstract
Carbazole-degrading bacterial strain B1 was isolated from an oil well sludge outlet in Qinghai Province.Degradation strain was screened by enriched culture,and analyzed,identified by physiological properties and 16S rDNA sequence,and the carbazole concentration of the culture was detected by HPLC.The degrading capacities of the strain under different pH,salt concentration and temperature,and the effect of adding carbon,nitrogen sources,and substrate concentration on degradation efficiency were discussed.Through identification,strain B1 was belonged to Sphingosinicella sp..The 100 mg/L carbazole degradation rate of B1 in 72 h under optimum conditions 30℃ and 7.0 pH could reach 98％,while its degradation rate could be higher when salt concentration was less than 10 g/L.Besides,the degradation rate was significantly improved by adding 0.1 g/L glucose and ammonium sulfate,and the strain B1 could tolerate 700 mg/L carbazole concentration.The results indicated that strain B1 had highlyefficient carbazole degradation ability and favorable environmental adaptability.关键词
咔唑/生物降解/Sphingosinicella sp./环境因素Key words
carbazole/biodegradation/Sphingosinicella sp./environmental factor分类
资源环境引用本文复制引用
边晨凯,张宇,陈度宇,许雷..一株咔唑降解菌的鉴定及其降解特性研究[J].中国农业科技导报,2017,19(5):51-59,9.基金项目
国家863计划项目(2006AA10C413)资助. （2006AA10C413）
