中国全科医学2017，Vol.20Issue(14)：1702-1706,5.DOI:10.3969/j.issn.1007-9572.2017.14.011
缺氧对肝泡状棘球蚴原头节血管内皮生长因子和CD34表达的影响研究
Effect of Anoxia on Expression of Vascular Endothelial Growth Factor and CD34 in Hepatic Alveolar Echinococcosis Protoscolex
摘要
Abstract
Objective To investigate the expression level of vascular endothelial growth factor(VEGF) and CD34 in the hepatic alveolar echinococcosis protoscolex under the condition of vitro anoxia.Methods From April to October in 2016,the 30 cases gerbils of abdominal infection with hepatic alveolar echinococcosis for 18 weeks were selected for experiment,and were sacrificed by cervical dislocation.The smooth clusters of hepatic alveolar echinococcosis tissues isolated in abdominal cavity or tissues adhere to the abdominal wall,mesentery,liver surface were selected.The hepatic alveolar echinococcosis protoscolex was cultured to divide into control group and experiment group(RPMI 1640 medium containing 100 μmol/L cobalt chloride).The hepatic alveolar echinococcosis protoscolex activity was observed by inverted microscope at the beginning,and the 6th,12th,18th,24th hour of the culture process.At the 12th and 24th hour of the culture process,enzyme-linked immunosorbent assay(ELISA) was used to detect the expression level of hypoxia-inducible factor 1 alpha(HIF-1α),VEGF and CD34 in hepatic alveolar echinococcosis protoscolex.Western blotting method was applied to detect the expression of VEGF and CD34.Results The echinococcosis protoscolex activity at the 0th,6th,12th,18th,24th hour of the culture process was not significantly different between control group and experiment group(P>0.05).Compared with the control group,the expression level of HIF-1α,VEGF,CD34 were significantly increased at the 12th and 24th hour of the culture process in the experiment group(P<0.05).Conclusion Hypoxia can increase the expression level of VEGF and CD34 in echinococcosis protoscolex,and this may be one of the important mechanisms of invasive growth presented by hepatic alveolar echinococcosis.关键词
棘球蚴病,肝/缺氧/血管内皮生长因子类/CD34Key words
Echinococcosis,hepatic/Anoxia/Vascular endothelial growth factors/CD34分类
医药卫生引用本文复制引用
张怀,张示杰,陈骞,王岩,苏争明,吴向未,孙红..缺氧对肝泡状棘球蚴原头节血管内皮生长因子和CD34表达的影响研究[J].中国全科医学,2017,20(14):1702-1706,5.基金项目
国家自然科学基金资助项目(81560518) （81560518）
