中国全科医学2017，Vol.20Issue(15)：1878-1883,6.DOI:10.3969/j.issn.1007-9572.2017.15.018
秦艽不同配伍药对对风寒湿痹类风湿关节炎模型大鼠血清炎性因子及踝关节病理学改变的影响
Effects of Qinjiao in Different Combinations on the Content of Serum Inflammatory Cytokines and Ankle Pathology in Rats with Wind-cold-damp Rheumatoid Arthritis
摘要
Abstract
Objective To elaborate the correlation of traditional Chinese medicine(TCM) properties with syndrome differentiation and treatment effect by observing the effects of Qinjiao(Gentianae Macrophyllae Radix) in different combinations on the content of serum inflammatory cytokines and ankle pathology in rats with wind-cold-damp rheumatoid arthritis(RA).Methods This study was conducted between March and December 2015.Eighty SPF grade SD rats were randomly divided into the blank group,collagenⅡmodel group,wind-cold-damp arthralgia model group,Tripterygium group,single Qinjiao group,Qinjiao-Weilingxian(Clematidis Radix et Rhizoma) group(hereinafter referred to as Qin-Wei group),Qinjiao-Sangjisheng(Taxilli Herba) group(hereinafter referred to as Qin-Sang group),Qinjiao-Fangji(Stephaniae Tetrandrae Radix) group(hereinafter referred to as Qin-Fang group).For modeling,the collagenⅡmodel group was induced by collagenⅡemulsion,other model groups except the blank model group were induced by collageⅡ and exposed in wind-cold-damp environment.After modeling,the blank group,collagenⅡmodel group,wind-cold-damp arthralgia model group were given the same amount of 0.9% sodium chloride solution,each of the other groups was gavaged with 15 ml/kg corresponding drug solution,once a day with administration duration of 30 min,for 28 days.The changes of general condition,paw swelling and arthritis index(AI) were observed.The serum levels of TNF-α and IL-1β were determined.Pathological changes of ankle joint were detected by HE staining.Results Specifically,compared with the blank group,the collagenⅡmodel group and wind-cold-damp arthralgia model group had higher average AI scores (P<0.05);the average AI scores in Tripterygium group,single Qinjiao group,Qin-Wei group,Qin-Sang group,Qin-Fang group were lower than those in the collagenⅡmodel group and wind-cold-damp arthralgia model group(P<0.05);the average AI scores of Qin-Wei group were significantly lower than those of single Qinjiao group,Qin-Sang group and Qin-Fang group(P<0.05).The serum TNF-α levels in the wind-cold-damp arthralgia model group were higher than those in blank group,collageⅡmodel group,Tripterygium group,single Qinjiao group and Qin-Wei group(P<0.05);the serum TNF-α levels in single Qinjiao group and Qin-Wei group were lower than those in Qin-Fang group(P<0.05).The serum IL-1β levels in collageⅡmodel group and wind-cold-damp arthralgia model group were higher than those in the blank group(P<0.05);the serum IL-1β levels in Tripterygium group,single Qinjiao group,Qin-Wei group,Qin-Sang group except Qin-Fang group were lower than those in collageⅡmodel group(P<0.05);Tripterygium group,single Qinjiao group,Qin-Wei group,Qin-Sang group and Qin-Fang group had lower serum IL-1β levels than wind-cold-damp arthralgia model group(P<0.05).Pathological changes of ankle joint detected by HE staining showed that,the inflammation and pathological destruction were alleviated in Tripterygium group,single Qinjiao group,Qin-Wei group,Qin-Sang group and Qin-Fang group in varying degrees,joint tissue morphology improved better in Qin-Wei group,and pathological changes improved the poorest in Qin-Fang group.Conclusion For the wind-cold-damp RA,herb with mild property combined with that with warm property,has better effect than two kinds of herbs with mild properties,and herb with mild property combined with that with cold property,and single herb does.The experimental results are consistent with the clinical treatment principles of TCM,"treating the cold disease should use the hot medicine".The mechanism of Qinjiao-Weilingxian compatibility having good effect on the disease may be related to its ability,which can reduce the serum TNF-α and IL-1β levels as well as synovial hyperplasia and cartilage destruction.关键词
关节炎,类风湿/模型,动物/肿瘤坏死因子α/踝关节/风寒湿痹Key words
Arthritis,rheumatoid/Models,animal/Tumor necrosis factor-alpha/Ankle joint/Wind-cold-damp arthralgia分类
医药卫生引用本文复制引用
马腾茂,强宇靖,罗奎元,刘飞,吴国泰,高慧琴..秦艽不同配伍药对对风寒湿痹类风湿关节炎模型大鼠血清炎性因子及踝关节病理学改变的影响[J].中国全科医学,2017,20(15):1878-1883,6.基金项目
国家自然科学基金资助项目(81360648) （81360648）
