中国畜牧兽医2017，Vol.44Issue(4)：941-949,9.DOI:10.16431/j.cnki.1671-7236.2017.04.009
脂肪酸结合蛋白5对奶牛乳腺上皮细胞乳脂合成的影响
Effect of Fatty Acid Binding Protein 5 on Milk Fat Synthesis of Bovine Mammary Epithelial Cells
摘要
Abstract
The fatty acid binding protein 5 (FABP5) is an intracellular lipid carrier.The TG GPO-POD assay kit and BODIPY staining methods were used to detect lipid secretion and triglyceride content,and the effect of FABP5 on SREBP-1c expression were detected by Real-time PCR and Western blotting methods.The results showed that high purity bovine mammary epithelial cells (BMECs) were successfully isolated and purified,and the eukaryotic expression vector pGCMV-IRES-EGFP-FABP5 was constructed in this experiment.Compared with the blank control group and empty vector group,the lipid secretion and triglyceride content, and the expression of SREBP-1c and FAS,ACC were extremely significantly increased when the FABP5 was overexpressed (P<0.01).FABP5 siRNA1 was selected as the optimal interference fragment, and when FABP5 was inhibited,the lipid secretion and triglyceride content,the expression of SREBP-1c,FAS and ACC were extremely significantly decreased (P<0.01).The results indicated that FABP5 could promote the synthesis of milk fat in BMEC by up-regulating the expression of SREBP-1c.关键词
奶牛乳腺上皮细胞/脂肪酸结合蛋白5/固醇调节元件结合蛋白-1c/乳脂Key words
bovine mammary epithelial cells (BMEC)/FABP5/SREBP-1c/milk fat分类
农业科技引用本文复制引用
于萌萌,李萌,李姗姗,李冬,高学军..脂肪酸结合蛋白5对奶牛乳腺上皮细胞乳脂合成的影响[J].中国畜牧兽医,2017,44(4):941-949,9.基金项目
国家高技术研究发展计划(863计划)项目(2013AA102504-03) （863计划）
