中国畜牧兽医2017，Vol.44Issue(4)：950-958,9.DOI:10.16431/j.cnki.1671-7236.2017.04.002
江苏省羊口疮病毒的分离鉴定及B2L基因的遗传进化分析
Isolation and Identification of Orf Virus from Jiangsu Province and Phylogenetic Analysis of Their B2L Gene
摘要
Abstract
The study was aimed to investigate prevalence of Orf virus (ORFV) in Jiangsu province in recent years and control Orf better.A total of 121 tissue samples were collected in some farms from 2013 to 2015 and subjected to PCR detection, viral isolation and phylogenetic analysis of B2L gene.Four samples were ORFV positive by PCR.The viruses were isolated by passaging in ovine fetal turbinate (OFTu) cells and MDBK cells, and were named as ORFV/Ovis/XZ/Jiangsu/2015/China, ORFV1/Ovis/DT/Jiangsu/2015/China, ORFV2/Ovis/DT/Jiangsu/2015/China and ORFV/Ovis/SL/Jiangsu/2015/China,respectively.The B2L gene was amplified and sequenced for the phylogenetic study.The nucleotide homology of these 4 strains was 98.5% to 100.0%.ORFV/Ovis/XZ/Jiangsu/2015/China, ORFV1/Ovis/DT/Jiangsu/2015/China and ORFV2/Ovis/DT/Jiangsu/2015/China, gathered into a cluster with SC-JY, GX-YB, JS-FX isolates and the nucleic acid homology of these strains was 97.8% to 100%.ORFV/Ovis/SL/Jiangsu/2015/China gathered into a cluster with LiaoNing, HuB and Gansu isolates, the nucleic acid homology was 98.8% to 98.9%.The nucleic acid homologies of 4 strains and ORFV strain China vaccine was 96.8% to 98.1%.The result showed that the ORFVs in Jiangsu province might be from different source.For controlling the spreading of this virus, it was necessary to carry out deep epidemiological survey in Jiangsu province.关键词
羊口疮病毒/胎羊鼻甲骨细胞/MDBK细胞/B2L基因/遗传进化分析Key words
Orf virus/OFTu cell/MDBK cell/B2L gene/phylogenetic analysis分类
农业科技引用本文复制引用
王秋霞,朱相儒,易成功,秦涛,陈素娟,彭大新,夏同海..江苏省羊口疮病毒的分离鉴定及B2L基因的遗传进化分析[J].中国畜牧兽医,2017,44(4):950-958,9.基金项目
苏北科技专项资金(富民强县)项目(BN2014002) （富民强县）
扬州大学校科研创新项目(x2015718) （x2015718）
江苏高校优势学科建设工程项目 （）
江苏高校品牌专业建设工程资助项目 （）
