中国畜牧兽医2017，Vol.44Issue(4)：959-964,6.DOI:10.16431/j.cnki.1671-7236.2017.04.003
MUC4和MUC13基因在ETECF18抗性型和易感型梅山断奶仔猪间的差异表达分析
Differential Expression Analysis of MUC4 and MUC13 Genes Between Resistant and Sensitive Weaned Meishan Piglets to ETEC F18
摘要
Abstract
MUC4 and MUC13 genes as important candidate genes for enterotoxigenic Escherichia coil (ETEC) F4 resistance,may play an important role in the process of against ETEC F18 infection in weaned piglets.In this study,ETEC F18-resistant and-sensitive weaned Meishan piglets were used,and the expression levels of MUC4 and MUC13 genes in 11 tissues (heart,liver,spleen,lung,kidney,stomach,muscle,thymus,lymph nodes,duodenum and jejunum) were determined by quantitative Real-time PCR.The results showed that MUC4 and MUC13 genes were broadly expressed with different expression levels in all the 11 tissues.In the thymus and lymph tissues,the expression of MUC4 gene in resistant piglets was significantly higher than that in sensitive piglets (P<0.05);In the lung tissue,the MUC13 gene expression level in resistant individuals was significantly higher than that in sensitive individuals (P<0.05),and in the intestinal tissues of duodenum and jejunum, the expression level of MUC13 gene was relatively higher in resistant individuals.Thus we speculated that the high expression of MUC4 gene in immune tissues and MUC13 gene in intestinal tissues might improve the immune ability of piglets,protect and lubricate the intestinal tract, and resist ETEC F18 infection.关键词
猪/MUC4基因/MUC13基因/产肠毒素大肠杆菌F18Key words
pig/MUC4 gene/MUC13 gene/ETEC F18分类
生物科学引用本文复制引用
钦伟云,甘丽娜,魏宗友,叶兰,包文斌,吴圣龙..MUC4和MUC13基因在ETECF18抗性型和易感型梅山断奶仔猪间的差异表达分析[J].中国畜牧兽医,2017,44(4):959-964,6.基金项目
江苏省科技支撑计划重点项目(BE2014357) （BE2014357）
苏州市科技局攻关项目(SNG201628) （SNG201628）
