中国畜牧兽医2017，Vol.44Issue(4)：965-972,8.DOI:10.16431/j.cnki.1671-7236.2017.04.004
马身猪MEF2A基因4种可变剪接体的克隆和生物信息学分析
Cloning and Bioinformatics Analysis of 4 Alternative Splice Variants of MEF2A Gene in Mashen Pig
摘要
Abstract
This study was aimed to clone the different splicing variants of myocyte enhancer factor 2A (MEF2A) gene in Mashen pig.According to the prediction results of the alternative splicing in the RNA-Seq sequencing, the coding region of MEF2A gene exons 5-8 were amplified.We analyzed the sequence characteristics of different splicing variants of MEF2A gene in Mashen pig, predicted conservative structure domain, constructed phylogenetic tree and compared the homology of MEF2A amino acid in different species by bioinformatics softwares.The results showed that 4 alternative splice variants of MEF2A gene were obtained.The exons 5-8 of MEF2A1 spliced normally.MEF2A2 lacked the exon 5, and the 5′end of exon 6 was 138 bp longer, the 3′end of exon 7 was 102 bp longer.MEF2A3 lacked exon 5, and the 5′end of exon 6 was 138 bp longer.The 3′end of MEF2A4 exon 7 of was 102 bp longer.The protein, encoded by inserted 138 bp sequence, contained a conserved domain-HJURP_C, which was the result of MEF2A participating in hepatocyte fibrosis.The MEF2A1, MEF2A4 and MEF2A1 of pig submitted in GenBank (accession No.NP_001090890.1) belonged to a subgroup, homology up to 98.9%.The MEF2A2, MEF2A3 and MEF2A2 of pig submitted in GenBank (accession No.NP_001093168.1) belonged to a subgroup, the homology was 98.2% and 98.9%, respectively.The successful cloning of 4 alternative splice variants of MEF2A gene laid the foundation for further study on the function of MEF2A protein.关键词
马身猪/MEF2A基因/可变剪接/生物信息学Key words
Mashen pig/MEF2A gene/alternative splice/bioinformatics分类
生物科学引用本文复制引用
张旗,李步高,郭晓红,高鹏飞,靳玉舒,李萌,成志敏,张宁芳,乐宝玉,曹果清..马身猪MEF2A基因4种可变剪接体的克隆和生物信息学分析[J].中国畜牧兽医,2017,44(4):965-972,8.基金项目
山西省科技创新重点团队项目(201605D131045-24) （201605D131045-24）
三晋学者项目(2016) （2016）
山西省农业科技攻关项目(20140311020-5) （20140311020-5）
山西省高等学校优秀青年学术带头人项目 （）
