中国畜牧兽医2017，Vol.44Issue(4)：973-979,7.DOI:10.16431/j.cnki.1671-7236.2017.04.005
lncLER在卢氏绿壳蛋鸡肝脏中表达调控的研究
The Expression and Regulation of lncLER in Liver of Lushi Green-shelled-egg Chicken
摘要
Abstract
Long noncording RNA (lncRNA) played an important regulating role in many biological processes.The lncRNA expression profile of liver of pre-laying hens (20 weeks old) and peak-laying hens (30 weeks old) in Lushi Green-shelled-egg chicken were obtained by lncRNA-seq.A lncRNA, which was named lncLER and expressed highly in the peak-laying hens was singled out.To investigate the expression characteristics of lncLER in liver of chicken,reverse-transcript PCR and Real-time fluorescence quantitative PCR were used to analyze the temporal expression profile of lncLER.Both individual chicken and primary hepatocytes were used to look into the effects of estradiol on expression of the lncLER in vivo and in vitro.The results showed that lncLER was expressed differently in various tissues and it's expression level was higher in the liver of peak-laying hens.The expression of lncLER, both in individual chicken and in primary hepatocytes,was up-regulated by estradiol.This research paved the way for further studying on the biological functions of lncLER in chicken egg production.关键词
长链非编码RNA/卢氏绿壳蛋鸡/雌激素/肝脏/表达调控Key words
lncRNA/Lushi Green-shelled-egg chicken/estrogen/liver/expression and regulation分类
生物科学引用本文复制引用
顾真真,李红,刘乙,谭文波,任俊晓,李艳敏,李转见,田亚东,刘小军..lncLER在卢氏绿壳蛋鸡肝脏中表达调控的研究[J].中国畜牧兽医,2017,44(4):973-979,7.基金项目
河南省国际合作项目(162102410030) （162102410030）
地方鸡种质资源保护与利用教育部创新团队发展计划(IRT16R23) （IRT16R23）
国家蛋鸡产业技术体系遗传育种岗位专项资金(CARS-41-K04) （CARS-41-K04）
