中国畜牧兽医2017，Vol.44Issue(4)：980-985,6.DOI:10.16431/j.cnki.1671-7236.2017.04.006
樱桃谷鸭源鹅出血性多瘤病毒VP3基因的克隆与序列分析
Cloning and Sequence Analysis of VP3 Gene of Cherry Valley Duck-origin Goose Hemorrhagic Polyomavirus
摘要
Abstract
In order to investigate goose hemorrhagic polyomavirus (GHPV) infection status in Fujian, China, 19 samples which were collected from different duck species were used to detecte GHPV, only one sample from Cheery Valley duck was tested positive (designated GHPV-FJ201601 strain).VP3 gene specific primers were designed by comparing the characterization of GHPV genomic sequences based on the genomic sequences download from GenBank.The length of the cloned VP3 gene was 654 bp, coding 217 amino acids.The theoretical pI of VP3 protein was 9.37, with 23 total number of negatively charged residues (Asp + Glu) and 28 total number of positively charged residues (Arg + Lys), respectively.The instability index of VP3 protein was 38.43, and it was a stable protein;The fat coefficient was 64.33;The total average hydrophobic index was-0.744.Also, the VP3 protein did not have signal peptides which was located in nucleus and having 14 phosphorylation sites.Nucleotides homology analysis of VP3 gene of GHPV isolates were higher with each other, which were no less than 99.7%.Phylogenetic analysis based on the VP3 gene showed that GHPV-FJ201601 strain shared the same genetic evolution cluster with goose-origin GHPV (14234 strain from Hungary and Toulouse Goose 2000 strain from France) rather than the duck-origin GHPV (Toulouse Muscovy Duck 2008 strain and Toulouse Mule Duck 2008 from France and 106 strain from China), all VP3 gene shared closer genetic distance with each other.In conclusion, this study was first evidence of Cheery Valley duck could infect GHPV in Fujian, which laid a good foundation for the enrichment the GHPV epidemiology data from different area and host.关键词
鹅出血性多瘤病毒/樱桃谷鸭/VP3基因/克隆/序列分析Key words
goose hemorrhagic polyomavirus/Cherry Valley duck/VP3 gene/clone/sequence analysis分类
农业科技引用本文复制引用
万春和,刘荣昌,程龙飞,傅光华,傅秋玲,施少华,陈红梅,黄瑜..樱桃谷鸭源鹅出血性多瘤病毒VP3基因的克隆与序列分析[J].中国畜牧兽医,2017,44(4):980-985,6.基金项目
国家水禽产业技术体系(CARS-43) （CARS-43）
国家自然科学基金(31602068) （31602068）
福建省畜禽疫病防控技术重大研发平台(2014N2003) （2014N2003）
福建省农业科学院畜牧兽医研究所基金(MYQJ2015(S)-3) （MYQJ2015(S）
福建省属公益类项目(2015R1023-7) （2015R1023-7）
福建省自然科学基金(2015J01114) （2015J01114）
