中国畜牧兽医2017，Vol.44Issue(4)：986-993,8.DOI:10.16431/j.cnki.1671-7236.2017.04.007
一周岁内滩羊皮肤差异表达蛋白研究
Study on Skin Differential Expression Proteins of Tan Sheep Within One Year Old
摘要
Abstract
In order to study different expressed proteins of one-year-old Tan sheep skin, two-dimensional gel electrophoresis (2-DE) was used to separate the Tan sheep skin proteins of different growth stages by Coomassie blue staining, with PDQuest 8.0 software to detect and analyze differences in protein spots,and were identified by MALDITOF/TOF-MS/MS.Protein maps of four growth stages were performed between pairwise, after testing found a total of 19 differentially expressed proteins, mass spectrometry successfully identified 15 protein spots and a total of 13 proteins, of which 14-3-3 σ protein isoforms, annexin A2, keratin 25 and tubulin α chain might be related to growth regulation sheep skin.Those different expressed proteins might be associated with different stages of growth of Tan sheep skin follicles cyclical changes.The different proteins of one-year-old Tan sheep skin provided theoretical basis for annual variation of Tan sheep skin.关键词
滩羊/皮肤/双向电泳/差异蛋白Key words
Tan sheep/skin/two-dimensional electrophoresis/differential proteins分类
农业科技引用本文复制引用
杨佐青,陶金忠,陈信,马瑞华,张鑫荣..一周岁内滩羊皮肤差异表达蛋白研究[J].中国畜牧兽医,2017,44(4):986-993,8.基金项目
宁夏农业育种专项"滩羊品系选育"项目(2013NYYZ0402) （2013NYYZ0402）
