中国畜牧兽医2017，Vol.44Issue(4)：1122-1127,6.DOI:10.16431/j.cnki.1671-7236.2017.04.026
猪Leptin基因多态性及其与产仔性状的相关性分析
The Association Analysis Between the SNPs of Leptin Gene and Litter Size Trait in Pigs
摘要
Abstract
The research was aimed to analyze the association of Leptin gene and breed traits on pigs.Three pairs of primers were designed which based on DNA sequence of pig Leptin gene (GenBank accession No.U66254, AF026976),the single nucleotide polymorphisms (SNPs) of Leptin gene were detected by PCR-SSCP in Sujiang and Jiangquhai pigs.The results showed that three genotypes of AA,AB and BB were identified, and the mutation (T3469C) in CDS of Leptin gene was found,but amino acid change weren't happened.The Chi-square test results showed that the Leptin genotype distribution of Sujiang and Jiangquhai pigs was in Hardy-Weinberg equilibrium (P>0.05).The polymorphism information content (PIC) were moderate polymorphism in Sujiang pigs,and low polymorphism in Jiangquhai pigs.The statistic analysis results showed that there were no significant association between the SNP of Leptin genotype and litter size trait(P>0.05).But the average litter size of T3469C site AB genotype was higher than AA and BB genotypes, it was concluded that the T3469C polymorphism of Leptin gene might be the genetic marker which potentially affected pig breed traits.关键词
苏姜猪/姜曲海猪/Leptin基因/PCR-SSCP/产仔性状Key words
Sujiang pigs/Jiangquhai pigs/Leptin gene/PCR-SSCP/litter size trait分类
农业科技引用本文复制引用
朱爱文,周春宝,倪黎纲,韩大勇..猪Leptin基因多态性及其与产仔性状的相关性分析[J].中国畜牧兽医,2017,44(4):1122-1127,6.基金项目
江苏省农业三新工程项目(SXGC[2014]271) （SXGC[2014]271）
江苏农牧科技职业学院青年科研基金(QN1214) （QN1214）
江苏高校品牌专业建设工程资助项目(PPZY2015A083) （PPZY2015A083）
