中国畜牧兽医2017，Vol.44Issue(4)：1175-1181,7.DOI:10.16431/j.cnki.1671-7236.2017.04.033
猪伪狂犬病病毒gB蛋白表达及免疫原性分析
Expression and Immunogenicity Analysis of Porcine Pseudorabies Virus gB Protein
摘要
Abstract
In order to study the expression and immunogenicity of porcine pseudorabies virus (PRV) gB protein,the specific primers were designed with the template of PRV preserved in the laboratory, and the 612 bp conserved gene fragments were amplified and sequenced, then it was cloned into the expression vector pET-28a and transformed into E.coli BL21 (DE3), the target protein was obtained after induced expression and purification.Western blotting was performed to analyse its immunogenicity.The results showed that gB protein was 30 ku, which mainly expressed in the form of inclusion body, and the concentration of the protein was 106 μg/mL, with well reactogenicity.13 PRV positive serum and 16 negative serum in the samples were detected using ELISA Kit on sale, using positive serum, the PRV antibody detection method was initially established with the PRV gB protein as antigen package.关键词
猪伪狂犬病病毒/gB蛋白/原核表达/免疫原性Key words
porcine pseudorabies virus/gB protein/prokaryotic expression/immunogenicity分类
农业科技引用本文复制引用
贾刚,樊梅娜,谷巍..猪伪狂犬病病毒gB蛋白表达及免疫原性分析[J].中国畜牧兽医,2017,44(4):1175-1181,7.基金项目
国家863计划专项基金(新型抗感染功能型微生态制剂的研制与应用)(2013AA102806) （新型抗感染功能型微生态制剂的研制与应用）
