中国畜牧兽医2017，Vol.44Issue(4)：1195-1202,8.DOI:10.16431/j.cnki.1671-7236.2017.04.036
一株凝结芽孢杆菌的分离鉴定及益生性研究
Study on the Isolation, Identification and Probiotic Characteristics of a Bacillus coagulans
摘要
Abstract
This experiment was conducted to isolate and identify a strain of Bacillus coagulans from the soil at the bottom of the pond, and study its probiotic properties.A strong acid producing bacillus was isolated from the soil at the bottom of the pond and identified as Bacillus coagulans through the physiological and biochemical characteristics and 16S rDNA sequence analysis.The strain had high tolerance to the simulated gastric juice (pH 2.5, 12 h of incubation, survival rate was 71.9%) and simulated intestinal fluid (bile salt concentration 0.3%, 12 h of incubation, survival rate was 84.4%) and had high antibacterial activity to E.coli K88, E.coli K99, S.agalactiae and A.hydrophila.It could degrade nitrite effectively and the degradation rate was 96.80% in 48 h.Two hundred and seventy AA broilers aged 1 day were randomly allocated into 3 groups.Diets were added 0 (group Ⅰ), 1.0×106 (group Ⅱ) and 1.0×107 CFU/g (group Ⅲ) Bacillus coagulans NJ102, respectively.The experiment lasted for 42 d.The average daily gain and final body weight of group Ⅱ and Ⅲ were significantly higher than that of group Ⅰ(P<0.05), and the feed weight ratio of the two groups were significantly lower than that of groupⅠ(P<0.05).There was no significant difference in average feed intake among three groups (P>0.05).It was found that the soil-derived Bacillus coagulans NJ102 from the bottom of the pond had a certain degree of resistance and potential probiotic ability, which could improve the growth performance of broilers and had the potential to be a microbial feed additive.关键词
凝结芽孢杆菌/分离/鉴定/底泥/益生菌/肉鸡Key words
Bacillus coagulans/isolation/identification/bottom soil/probiotic/broiler分类
农业科技引用本文复制引用
汪攀,易敢峰,孙自博,王蕊,陈丽仙,夏冬梅..一株凝结芽孢杆菌的分离鉴定及益生性研究[J].中国畜牧兽医,2017,44(4):1195-1202,8.基金项目
北京科委科技创新基地培育与发展工程子专项(Z161100005016086) （Z161100005016086）
大北农集团自立研发项目(DBN20150001) （DBN20150001）
