中国烟草学报2017，Vol.23Issue(2)：78-83,6.DOI:10.16472/j.chinatobacco.2016.281
普通烟草长叶柄突变性状的遗传分析
Genetic analysis of long petiole mutant traits in Nicotiana tabacum L.
摘要
Abstract
Genetic analysis of long petiole traits of Nicotiana tabacum L.was conducted to study the development of tobacco petiole and leaf.A long and very long petioles mutants (M48,M50) whose wild type had no petioles was obtained respectively from Nicotiana tobacum L.varieties Zhongyan100 (ZY) and Honghuadajinyuan (HD) by EMS mutagenesis.The long petiole mutants were hybridized with their own wild type Zhongyan100 and Honghuadajinyuan respectively,as well as Nicotiana tobacum L.variety Gexinsanhao (G3),resulting in the formation of F1.The F1 was self-crossed to obtain F2 population and were then backcrossed to respective wild types to get BC 1F1 population.Phenotypic investigation and statistical analysis results of all materials showed that F1 was all characterized by a (very) long petiole mutation phenotype.Theoretical separation ratio of mutant phenotypes and wild type phenotypes conformed to 3∶1 and 1∶1 by the chi square test in F2 and BC1F1 populations.It was suggested that the trait of (very) long petiole mutant was controlled by a pair of dominant single gene on the chromosome.These results laid solid foundation for further (very) long petiole mutant gene mapping and cloning,revealing its role in the development of tobacco petiole and leaf.关键词
普通烟草/EMS诱变/叶柄突变/遗传分析Key words
Nicotiana tobacum L./EMS mutagenesis/petiole mutant/genetic analysis引用本文复制引用
陈果,王元英,王绍美,吴新儒,屈旭,晁江涛,向小华,胡军华,陈浣,王新,刘贯山..普通烟草长叶柄突变性状的遗传分析[J].中国烟草学报,2017,23(2):78-83,6.基金项目
国家烟草专卖局烟草基因组计划重大专项项目[110201301005(JY-05)] （JY-05）
