中国烟草学报2017，Vol.23Issue(2)：92-96,5.DOI:10.16472/j.chinatobacco.2016.354
一个与烟草TMV抗性基因N紧密连锁的共显性SSR标记
Co-dominant SSR marker closely linked to TMV resistance gene N of tobacco (Nicotiana tobaccum L.)
摘要
Abstract
Tobacco mosaic virus (TMV) was one of the major diseases in tobacco (Nicotiana tabaccum L.) production.Separate progenies (BC1F1 population) from a cross between a resistant parent Coker176 and susceptible parent Y3 were used to establish TMV resistance gene N molecular marker-assisted selection system by bulked segregation analysis (BSA) method and simple sequence repeat (SSR) technology techniques.Among 18,764 SSR primer pairs,396 SSR primers were polymorphic between parents,while only one SSR marker TM508-007 amplified products was closely linked to TMV-resistant gene N and the genetic distance between TM508-007 and gene N was 0.41 cm.The co-dominant SSR marker screened in this study enriched the number and types of molecular markers in terms of tobacco's resistance to TMV,and they are expected to be used as marker-assisted selection in tobacco's resistance to TMV disease.关键词
烟草/烟草花叶病/分离集团分组分析法/共显性SSR标记Key words
Tobacco (Nicotiana tabccum L.)/tobacco mosaic virus (TMV)/bulked segregation analysis (BSA)/co-dominant SSR marker引用本文复制引用
粟阳萌,童治军,李梅云,焦芳婵,耿素祥,李永平..一个与烟草TMV抗性基因N紧密连锁的共显性SSR标记[J].中国烟草学报,2017,23(2):92-96,5.基金项目
中国烟草总公司云南省公司科技项目(2016YN27) （2016YN27）
