中国药理学与毒理学杂志2017，Vol.31Issue(5)：414-421,8.DOI:10.3867/j.issn.1000-3002.2017.05.006
芫花素对模式生物秀丽隐杆线虫的毒性作用
Toxic effect of genkwanin on model organism Caenorhabditis elegans
摘要
Abstract
OBJECTIVE To explore the toxicity and mechanisms of genkwanin, one of the main components of Flos Genkwa, using the model organism Caenorhabditis elegans (C. elegans) assay system. METHODS Both wild-type and transgenic strains of C. elegans were treated with genkwanin 0.1, 1, 10 and 100 μmol · L-1 for 24, 48 and 72 h, respectively. The head thrashes, body bends, lifespan, brood size, mean defecation cycle length and pumping behavior of the wild-type C. elegans as well as the fluo?rescence intensity and the number of GABAergic neurons of the transgenic C. elegans were observed under microscopes. RESULTS ① Wild-type C. elegans:Compared with the normal control group, after treatment with genkwanin for 24 h, the times of head thrashes and body bends of C.elegans showed significant decreases (P<0.05, P<0.01) in genkwanin 1-100μmol·L-1 groups. After treatment with genk?wanin for 48 h, the times of body bends showed a significant decrease (P<0.05) in genkwanin 0.1μmol·L-1 group, the times of head thrashes and body bends showed a significant decrease (P<0.01) in genkwanin 1-100 μmol · L-1 groups, but a significant increase of mean defecation cycle length (P<0.05) and a decrease of pumping behavior (P<0.01) were observed in genkwanin 100μmol·L-1 groups. After treat?ment with genkwanin for 72 h, a decrease in the times of head thrashes and body bends (P<0.05, P<0.01), an increase of mean defecation cycle length (P<0.05, P<0.01), and a decrease of pumping behavior (P<0.05, P<0.01) in C. elegans were observed in genkwanin 0.1-100 μmol · L-1 groups. ② Transgenic C. elegans:Compared with the normal control, after treatment with genkwanin for 24 h, the fluores?cence intensity of GABAergic neurons of the transgenic C. elegans showed significant decreases (P<0.05, P<0.01) in genkwanin 1-100μmol · L-1 groups. After treatment with genkwanin for 48 h, the fluores?cence intensity of GABAergic neurons (P<0.05, P<0.01) and the number of VD neurons (P<0.01) showed significant decreases in genkwanin 1-100μmol·L-1 groups. After treatment with genkwanin for 72 h, the fluorescence intensity of GABAergic neurons (P<0.05, P<0.01) decreased in genkwanin 0.1-100μmol · L-1 groups, so did the number of AVL (P<0.05, P<0.01) and VD neurons (P<0.05, P<0.01) in genkwanin 1-100μmol·L-1 and 10-100μmol·L-1 groups, respectively. CONCLUSION Genkwanin has certain toxicity in C. elegans mainly by inhibiting the locomotion behaviors. The underlying mechanism may be related to the impairment of GABAergic neurons.关键词
秀丽隐杆线虫/芫花素/毒性Key words
Caenorhabditis elegans/genkwanin/toxicity分类
医药卫生引用本文复制引用
许云,阮秦莉,纪蕾蕾,嵇晶,吴晨曦,王梦..芫花素对模式生物秀丽隐杆线虫的毒性作用[J].中国药理学与毒理学杂志,2017,31(5):414-421,8.基金项目
国家自然科学基金(81202981) （81202981）
江苏省中药资源产业化过程协同创新中心开放课题(ZDXM-2-7) The project supported by National Natural Science Foundation of China (81202981) （ZDXM-2-7）
and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization (ZDXM-2-7) （ZDXM-2-7）
