中国肿瘤生物治疗杂志2017，Vol.24Issue(4)：341-347,7.DOI:10.3872/j.issn.1007-385X.2017.04.002
循环肿瘤细胞检测研究进展:从单纯计数到表型与功能分析
Progresses of research on detection of circulating tumor cells: from simple counting to analysis of phenotypes and function
摘要
Abstract
With development of novel techniques for targeted therapy,immunotherapy and so on,the tumor diagnosis and treatment have entered a new era of precision treatment.Liquid biopsy played an increasingly important role in the precision treatment of tumors as a convenient and noninvasive assay which could dynamically reflect the whole pictures of tumor gene spectrum.Detection of circulation tumor cells (CTCs) in blood is the earliest liquid biopsy technique and has been widely used in the early diagnosis,prognosis judgment,efficacy evaluation and so on for tumors.At primary stage,the detection of CTCs mainly based on a purely counting.However,a fact that the purely counting cannot really reflect the situations of tumor load and changes was gradually found.With continuous developments of sequencing technique and so on for a single cell,understanding of CTCs is also deepening and the detection of CTCs gradually turned to the advanced stage that is based on analysis of molecular phenotype and function.This paper mainly elaborated a development of CTCs detection from a primary stage to an advanced one and progresses of the research on its application in the precision treatment of tumor,combining with clinical practice for recent years.关键词
循环肿瘤细胞/液体活检/细胞计数/分子表型/功能分析Key words
circulating tumor cell (CTCs)/liquid biopsy/simple counting/molecular phenotype/functional analysis分类
医药卫生引用本文复制引用
毕峰瑞,颜宏利..循环肿瘤细胞检测研究进展:从单纯计数到表型与功能分析[J].中国肿瘤生物治疗杂志,2017,24(4):341-347,7.基金项目
国家自然科学基金项目(No.81672350,No.81472770,No.81272280) （No.81672350,No.81472770,No.81272280）
上海市重点弱势学科支持项目-检验病理学科(No.2015ZB0202). Project supported by the National Natural Science Foundation of China(No.81672350,No.81472770,No.81272280),and the Key Developing Discipline of Shanghai (No.2015ZB0202) （No.2015ZB0202）
