中国肿瘤生物治疗杂志2017，Vol.24Issue(4)：348-354,7.DOI:10.3872/j.issn.1007-385X.2017.04.003
直肠癌循环肿瘤细胞与原发肿瘤组织基因表达谱的差异分析
Analysis of differentially expressed profiles of circulating tumor cells and primary tumor tissues in rectal cancer
摘要
Abstract
Objective:To screen specific gene of circulating tumor cells (CTCs) of rectal cancer according to the differentially expressed profile of rectal cancer CTCs and primary tumor tissues.Methods:Four cases of rectal cancer patients treated in Fujian Cancer Hospital from September 2015 to December 2016 were selected to collect tumor tissues and corresponding para-cancerous tissues,in addition,20 ml of peripheral blood was collected.Moflo XDP cytometry was adopted to identify and sort CTCs;after purification and amplification of sample total RNA,human whole transcriptome microarray chip was used to detect the expression profiles of CTCs and tumor tissue mRNA of corresponding patients,and further to screen the CTCs specific genes.In addition,MAC3.0 bio-informatics software was used for the gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.Results:After sorting and testing,CTCs number sorted from each patient were 67,78,53 and 120.There were 7 755 differentially expressed gene between tumor tissues and normal para-cancerous tissues;there were 567 differentially expressed gene between CTCs and white cells;Combining the two dataset,there were 36 differentially co-expressed gene,including 16 up-regulated genes and 20 down-regulated genes (P ＜ 0.05 or P ＜ 0.01).GO function of CTCs specific genes were correlated with tumor metastasis and stem cell function,and those specific genes also participated in 22 signaling pathways related to cell migration,stem cell function and immune function.Conclusion:Compared with primary tumor gene,CTCs has a unique mRNA profile,which is the foundation of tumor recurrence and metastasis.关键词
直肠癌/循环肿瘤细胞/基因表达谱Key words
rectal cancer/circulating tumor cells (CTCs)/gene expression profile分类
医药卫生引用本文复制引用
许扬梅,龚福生,陈路川,应敏刚,郑秋红..直肠癌循环肿瘤细胞与原发肿瘤组织基因表达谱的差异分析[J].中国肿瘤生物治疗杂志,2017,24(4):348-354,7.基金项目
福建省医学创新基金资助项目(No.2015-CXB-4) （No.2015-CXB-4）
福建省自然科学基金资助项目(No.2016J01436) （No.2016J01436）
国家临床重点专科建设基金资助项目(No.2015-544).Project supported by the Medical Innovation Project of Fujian Province(No.2015-CXB-4),the Natural Science Foundation of Fujian Province(No.2016J01436),and the National Clinical Key Specialty Construction Program(No.2015-544) （No.2015-544）
