中国肿瘤生物治疗杂志2017，Vol.24Issue(4)：389-394,6.DOI:10.3872/j.issn.1007-385X.2017.04.009
小干扰RNA沉默CXCR4和CXCR7对人子宫内膜癌Ishikawa细胞裸鼠移植瘤生长的抑制作用
Inhibition effect of siRNA silencing CXCR4 and CXCR7 on the growth of Ishikawa cell xenografts in nude mice
摘要
Abstract
Objective:To evaluate the effect of small interfering RNA (siRNA) of CXCR4 and CXCR7 on the growth of endometrial cancer (EC) Ishikawa cells xenografts in nude mice.Methods:EC Ishikawa cells were subcutaneously injected into the right flank of nude mice to establish animal xenograft model.When the diameter of tumors reached 5-7 mm,all tumor-bearing mice were randomly divided into five groups,and treated with intra-tumor injection of CXCR4-siRNA,CXCR7-siRNA,CXCR4-siRNA + CXCR7-siRNA,negative-siRNA,and normal saline,respectively.Growth of xenografts was observed,and the differences in volume and weight of xenografts among five groups were examined.CXCR4 and CXCR7 expressions in tumor xenografts were detected by quantitative reverse transcription polymerase chain reaction (QRT-PCR),Western blotting and immunohistochemical technique,respectively.Results:Ishikawa cells xenograft model in nude mice was successfully constructed.The growth of tumor xenografts in CXCR4 siRNA group,CXCR-siRNA group and CXCR4-siRNA + CXCR7-siRNA group were significantly inhibited,compared with those in negative control and blank control group (all P ＜ 0.05).The mRNA (P ＜ 0.05) and protein (P ＜ 0.01) expressions of CXCR4 and CXCR7 were statistically down-regulated by directly injecting siRNA into xenografts.Conclusion:siRNA interfering CXCR4 and CXCR7 could effectively suppress the growth of human EC Ishikawa xenografts in nude mice.关键词
siRNA/CXCR4/CXCR7/子宫内膜癌/裸鼠Key words
small interfering RNA(siRNA)/CXCR4/CXCR7/Endometrial carcinoma/nude mouse分类
医药卫生引用本文复制引用
叶园英,黄煜,颜莉莉,赵淑萍,张磊..小干扰RNA沉默CXCR4和CXCR7对人子宫内膜癌Ishikawa细胞裸鼠移植瘤生长的抑制作用[J].中国肿瘤生物治疗杂志,2017,24(4):389-394,6.基金项目
山东省优秀中青年科学家科研奖励基金资助项目(No.BS2009SW002),山东省自然科学基金资助项目(No.ZR2013HM012).Project supported by the Scientific Research Foundation of Shandong Province for Outstanding Young Scientist Award(No.BS2009SW002),and the Natural Science Foundation of Shandong Province(No.ZR2013HM012) （No.BS2009SW002）
