中国肿瘤生物治疗杂志2017，Vol.24Issue(4)：395-399,5.DOI:10.3872/j.issn.1007-385X.2017.04.010
金雀异黄素对肝癌Bel-7404细胞放疗增敏作用及其可能的机制
Genistein enhances radiosensitivity of hepatoma Bel-7404 cells and its possible mechanisms
摘要
Abstract
Objective:To investigate the effects and mechanisms of genistein (Gen) enhancing radio-sensitivity of Hepatoma Bel-7404 cells.Methods:Bel-7404 cells were divided into four groups:normal control (NC),genistein treatment (G),radiation alone treatment (R) as well as genistein + radiation treatment group (G + R).Except the NC group,the other three groups were treated with genistein (5 μmol/L) and/or X-ray irradiation (8 Gy) at a dose rate of 300 cGy/min,respectively.The effect of Gen and x-ray on cell proliferation,cell cycle and apoptosis of Bel-7404 cells were detected using CCK-8 and flow cytometry,respectively;the expression levels of ATM-Chk2 signal pathway related proteins were detected using Western blotting.Results:The inhibitory rate on cell proliferation in G + R group was significantly higher than that in R group at 24 h after irradiation ([16.80 ± 2.59] ％ vs [9.76 ± 2.11] ％,P ＜ 0.05).And the inhibitory effect was enhanced in a time-dependent manner.Compared with NC group,the cell apoptosis rate and percentage of cells in G2/M phase were all significantly increased in G,R and G + R group (P ＜ 0.05 or P ＜ 0.01).Combination of Gen with X-ray further increased cell apoptosis compared with X-ray treatment alone group ([51.86 ± 2.88] ％ vs [33.18 ± 4.48] ％,P ＜ 0.01).Furthermore,the expressions of p-ATM and p-Chk2 were obviously up-regulated in G + R group compared with that of R,G and NC group.Conclusions:Gen could enhance radiosensitivity of hepatoma Bel7404 cells,and the mechanism might be related with the activation of ATM-Chk2 signal pathway.关键词
金雀异黄素/放疗/肝癌/增敏Key words
genistein/radiation/sensitization/liver cancer分类
医药卫生引用本文复制引用
姜婧,颜宏利,杜爱英,郭玉玲,宋立华..金雀异黄素对肝癌Bel-7404细胞放疗增敏作用及其可能的机制[J].中国肿瘤生物治疗杂志,2017,24(4):395-399,5.基金项目
国家自然科学基金资助项目(No.31570869,31170844).Project supported by the National Natural Science Foundation of China Grants(No.81472770.81672350) （No.31570869,31170844）
