中国肿瘤生物治疗杂志2017，Vol.24Issue(4)：400-403,4.DOI:10.3872/j.issn.1007-385X.2017.04.011
青蒿琥酯对人肺癌A549细胞恶性生物学行为的影响及其可能机制
Effects and possible mechanisms of artesunate on the invasion and migratory ability of human lung cancer A549 cells
摘要
Abstract
Objective:To investigate the effects of artesunate (ART) on the proliferation,invasion and migratory ability of human lung cancer A549 cells and its possible mechanisms.Methods:CCK-8 assay was used to analyze the proliferation level of A549 cells after treatment with different concentrations of ART for 24 and 48 h.Transwell assay and wound healing assay were adopted to evaluate the invasion and migration of A549 cells after the treatment of ART at concentration of 30 μg/ml,respectively.Western blotting was used to detect the change in protein expressions of human antigen R (HuR) and MMP-9 in A549 cells after the treatment with ART (30 μg/ml).Results:ART inhibited the proliferation of human lung cancer A549 cells in a dose-dependent manner (P ＜ 0.05).Compared with untreated group,number of A549 cells penetrating the membrane in ART group (30μg/ml) was significantly decreased([47.11 ± 8.61] vs [131.00 ± 14.58],P ＜ 0.01),and the migration distance was remarkably reduced ([1.08 ± 0.13] vs [2.91 ± 0.24] mm,P ＜ 0.01).In addition,ART significantly inhibited the protein expression of HuR and MMP-9 in A549 cells (P ＜ 0.05).Conclusion:ART can inhibit the proliferation,invasion and migration of A549 cells,and this effect may be related to inhibition of HuR expression.关键词
青蒿琥酯/人肺癌A549细胞/人抗原R/迁移/侵袭Key words
artesunate/non-small cell lung cancer(NSCLC)/human antigen R(HuR)/migration/invasion分类
医药卫生引用本文复制引用
辛重阳,孙肖,王俊,王宝成..青蒿琥酯对人肺癌A549细胞恶性生物学行为的影响及其可能机制[J].中国肿瘤生物治疗杂志,2017,24(4):400-403,4.基金项目
国家自然科学基金资助项目(No.81572875,No.81272619).Project supported by the National Science Foundation of China(No.81572875,No.81272619) （No.81572875,No.81272619）
