中国中医药信息杂志2017，Vol.24Issue(6)：68-70,3.DOI:10.3969/j.issn.1005-5304.2017.06.017
HPLC测定不同产地北豆根中蝙蝠葛碱含量
Content Determination of Dauricine in Menispermi Rhizoma from Different Producing Areas by HPLC
摘要
Abstract
Objective To establish an HPLC method to determine the contents of dauricine in Menispermi Rhizoma from different producing areas. Methods C18 was set as chromatographic column filler, with acetonitrile-water-triethylamine (45:55:0.1) as the mobile phase, 284 nm as the ultraviolet wavelength detection, 1 mL/min as the flow rate, 30 ℃ as the column temperature. HPLC chromatograms of eight different batches of Menispermi Rhizoma were established. Results HPLC testing conditions of Menispermi Rhizoma was established. Within 20-100 μg/mL, there was a good linear relationship between the injection volume of the reference substance and the peak area (r=0.9995). The average recovery of dauricine was 100.30%, RSD=1.000%. The contents of dauricine in Menispermi Rhizoma from different producing areas were different. Conclusion The HPLC method is with sensitivity, accuracy, precision, good reproducibility and simple operation, which can be used as detection method to determine the content of dauricine in Menispermi Rhizoma.关键词
北豆根/蝙蝠葛碱/含量测定/高效液相色谱法Key words
Rhizoma Menispermi/dauricine/content determination/HPLC分类
医药卫生引用本文复制引用
杜憬生,杜楚玲,邵长丽,蔡宇,黄志海..HPLC测定不同产地北豆根中蝙蝠葛碱含量[J].中国中医药信息杂志,2017,24(6):68-70,3.基金项目
广东省科学技术计划(2012B031800200) （2012B031800200）
