中国中西医结合杂志2017，Vol.37Issue(4)：443-447,5.DOI:10.7661/j.cjim.20170203.012
β-细辛醚对胃癌裸鼠上皮间质转化的影响
Effects of β-asarone on Epithelial-Mesenchymal Transition of Gastric Cancer in Nude Mice
摘要
Abstract
Objective To observe the effects of β-asarone on epithelial-mesenchymal transition (EMT) of human gastric cancer MGC-803 cells in BALB/c nude mice,and to study its possible molecular mechanism.Methods Gastric cancer MGC-803 cells were subcutaneously inoculated to nude mice for preparing transplanted tumor model.Totally 24 nude mice were then divided into the negative control group (model),the positive control group (5-FU,25 mg/kg),the high dose β-asarone group (100 mg/ kg),the low dose β-asarone group (50 mg/kg),8 in each group.Corresponding medicines were administered to rats in respective group by gastrogavage,once per day for 10 successive days.The mice were sacrificed at the end of the intervention,and the tumor inhibition rate was calculated.The expressions of E-cadherin,N-cadherin,Snail,phosphatidylinositol 3-kinase (PI3K),phosphorylation of phosphatidylinositol 3-kinase (p-PI3K),serine/threonine kinase (AKT),phosphorylation of serine/threonine kinase (p-AKT) were detected by Real-time PCR and Western Blot.Results Compared with the model group,the volume of transplanted tumor was obviously reduced in 5-FU group and β-asarone groups from day7 to day 11 (P ＜0.05).Protein and mRNA expressions of N-cadherin,Snail,p-PI3K,p-AKT decreased,and protein and mRNA expressions of E-cadherin increased in 5-FU group and β-asarone groups (P ＜ 0.05).Conclusions β-asarone could inhibit proliferation ability of gastric cancer cells,and its mechanism might be associated with down-regulating PI3K/AKT signal pathway of gastric cancer cells and restraining EMT of gastric cancer cells.关键词
β-细辛醚/胃癌细胞/移植瘤/上皮间质转化/PI3K/AKT信号通路Key words
β-asarone/gastric cancer cell/transplanted tumor/epithelial-mesenchymal transition/PI3K/AKT signal pathway引用本文复制引用
吴坚,邹玺,陈敏,刘沈林,张星星..β-细辛醚对胃癌裸鼠上皮间质转化的影响[J].中国中西医结合杂志,2017,37(4):443-447,5.基金项目
国家自然科学基金资助项目(No.81473605) （No.81473605）
国家中医临床研究基地业务建设科研专项(No.JDZX2012086) （No.JDZX2012086）
江苏省高校优势学科建设工程一期资助项目 （）
江苏省中医院院级课题(No.Y14074) （No.Y14074）
江苏省临床医学科技专项(No.BL2014100) （No.BL2014100）
