中国中西医结合杂志2017，Vol.37Issue(4)：453-457,5.DOI:10.7661/j.cjim.20170207.014
复心合剂对心力衰竭大鼠β1-AR-cAMP-PKA通路的影响
Effect of Fuxin Mixture on The β1-AR-cAMP-PKA Pathway of Rats with Heart Failure
摘要
Abstract
Objective To observe the effect of Fuxin Mixture(FXM) on the β1-AR(adrenergic receptor)-cAMP(cyclic adenosine monophosphate,cAMP)-PKA (protein kinase A,PKA) pathway of rats with heart failure.Methods Male Wistar rats were randomly divided into blank control group,captopril group,FXM low dose group,FXM high dose group and model group.Models of CHF were established.After drug intervention for 6 weeks,the left ventricular mass index(LVMI) was analysed,the expression of β1-AR mRNA in myocardial tissue was measured,the level of cAMP in rat plasma,the OD value PKA content of spleen tissue homogenate were detected.Results Compared with the blank control group,the LVMI and the cAMP in plasma of model group were increased (P ＜0.05),the expression of β1-AR mRNA,the OD value of spleen tissue homogenate and PKA were decreased (P ＜0.01).Compared with the model group,the LVMI were decreased,and the expression of β1-AR mRNA were increased in FXM high dose group and captopril group (P ＜0.01,P ＜0.05);the level of cAMP in plasma of each drug group were decreased (P ＜0.01),the OD value of spleen tissue homogenate and PKA were increased (P ＜0.01).Compared with the captopril group,the expression of β1-AR mRNA,the OD value of spleen tissue homogenate and PKA were decreased,and the LVMI and the cAMP were increased in the FXM low dose group (P ＜0.01,P ＜0.05).Compared with the FXM low dose group,the LVMI and the cAMP of FXM high dose group were decreased (P ＜0.05),the expression of β1-AR mRNA,the OD value of spleen tissue homogenate and PKA were increased (P ＜0.01).Conclusion FXM could play the role of anti-heart failure through regulating β1-AR-cAMP-PKA pathway.关键词
复心合剂/心力衰竭/β1-AR-cAMP-PKA通路/大鼠Key words
Fuxin Mixture/chronic heart failure/β1-AR-cAMP-PKA pathway/rat引用本文复制引用
薛一涛,李焱,焦华琛,刘秀娟,鲁月凤..复心合剂对心力衰竭大鼠β1-AR-cAMP-PKA通路的影响[J].中国中西医结合杂志,2017,37(4):453-457,5.基金项目
国家自然科学基金资助项目(No.81273703) （No.81273703）
