中国中西医结合杂志2017，Vol.37Issue(4)：458-463,6.DOI:10.7661/j.cjim.20170207.015
藤莓汤对胶原诱导性关节炎大鼠滑膜PPARγ/NF-κB信号途径的影响
Effects of Tengmei Decoction on the PPARγ/NF-κB Signaling Pathway in Synovium of Collagen-induced Arthritis Rats
摘要
Abstract
Objective To observe the effects of Tengmei Decoction (TMD) on the expressions of peroxisome proliferator activated receptor gamma (PPARγ),nuclear factor kappa B (NF-κB),and IL-17 in synovium of collagen-induced arthritis (CIA) rats,and to study its molecular mechanism of inhibiting synovial immune inflammatory injuries.Methods CIA model was established in Sprague-Dawley rats.Successfully modeled rats were randomly divided into the model group,the positive drug group,high and low dose TMD groups,6 in each group.Besides,a normal group was set up (n =6).Deionized water (10 mL · kg-1 · d-1) was administrated to rats in the normal group and the model group by gastrogavage.Leflunomide (1.87 mg · kg-1 · d-1) was administrated to rats in the positive drug group by gastrogavage.TMD (31.8 g crude drugs · kg-1 · d-1 and 15.9 g crude drugs · kg-1 · d-1) was administrated to rats in high and low dose TMD groups respectively by gastrogavage.The intervention lasted for 12 successive weeks.Protein and mRNA levels of PPARγ,P65,and IL-17 were detected at the end of intervention.Results Compared with the normal group,mRNA and protein expression levels of PPARγ,P65,and IL-17 were up-regulated in the model group (P ＜0.01).Compared with the model group,PPARγprotein expression level was up-regulated,mRNA and protein expression levels of P65 and IL-17 were downregulated in high dose TMD group (P ＜0.01).mRNA and protein expression levels of PPARγ were upregulated,mRNA and protein expression levels of P65 and IL-17 were down-regulated in the positive drug group and low dose TMD group (P ＜0.01).Conclusions TMD could ameliorate pathological damage of joint synovium,and inhibit expressions of immune inflammatory factors.关键词
藤莓汤/胶原诱导性关节炎/滑膜/过氧化物酶体增殖物激活受体-γ/核转录因子-κBKey words
Tengmei Decoction/collagen-induced arthritis/synovium/peroxisome proliferator activated receptor gamma/nuclear factor kappa B引用本文复制引用
王洁,张建萍,陆清怡,孟凤仙,王亚南,陆妍,陈祎,顾文,王康,沈志明,卜祥伟,张红红..藤莓汤对胶原诱导性关节炎大鼠滑膜PPARγ/NF-κB信号途径的影响[J].中国中西医结合杂志,2017,37(4):458-463,6.基金项目
教育部高等学校博士学科专项科研基金(No.20120013110006) （No.20120013110006）
