中国中西医结合杂志2017，Vol.37Issue(4)：476-479,4.DOI:10.7661/j.cjim.20170204.018
山姜素对肺微血管内皮细胞损伤保护机制
Protection Mechanism of Alpinetin on Pulmonary Microvascular Endothelial Cells Injury
摘要
Abstract
Objective To explore the protection mechanisms about Alpinetin to acute human pulmonary microvascular endothelial cells (HPMECs) injury.Methods Different concentration of LPS (0.01,0.1,1.0,10 mg/L) was applied to the HPMECs to induce acute HPMECs injury.The HPMECs models (n =3) were intervened by Alpinetin(40,80,160,320 mg/L).Normal HPMECs were selected as control group.The viability of HPMECs was observed,mRNA and protein expression of intercellular cell adhesion molecule-1 (ICAM-1),TNF-α,APQ-1 were detected.Results Compared with control group,the protein expression of ICAM-1,TNF-α were increased,the protein and mRNA expression of APQ-1 and the viability of HPMECs were decreased in model group (P ＜0.05).Compared with model group,ICAM-1 and TNF-α protein expressions were significantly inhibited in Alpinetin(80,160 mg/L)group,the mRNA and protein expression of APQ-1 and the viability of HPMECs were significantly increased (P ＜0.05,P ＜ 0.01).Conclusion Alpenitin could protect the HPMECs injury by down-regulated protein expression of ICAM-1,TNF-α and up-regulated the mRNA and protein expression of APQ-1.关键词
山姜素/水通道蛋白/人肺微血管内皮细胞/损伤Key words
Alpinetin/APQ-1/HPMECs/injury引用本文复制引用
王振冉,李扬,唐博..山姜素对肺微血管内皮细胞损伤保护机制[J].中国中西医结合杂志,2017,37(4):476-479,4.基金项目
广西卫生厅中医药科技专项项目(No.GZPT13-45) （No.GZPT13-45）
