癌变·畸变·突变2017，Vol.29Issue(3)：194-198,204,6.DOI:10.3969/j.issn.1004-616x.2017.03.007
CCM3基因敲降斑马鱼模型的建立及其在毒理学中的初步应用
Establishment of a CCM3 gene knockdown zebrafish model and its preliminary application in toxicology
摘要
Abstract
OBJECTIVE: To establishment a zebrafish model with CCM3 gene knockdown and to investigate its usefulness as a biological monitoring model for low concentrations of cardiovascular toxicants in water. METHODS:The transgenic zebrafish line (VEGFR2:GFP) which expresses green flurescence proteins in blood vessels were used. The one-cell stage zebrafish eggs were injected with morpholino oligos (MO) at indicated doses. Laser scanning confocal microscopy was used to observe the intracranial vessels of zebrafish at 72 h. The zebrafish model with CCM3 gene knockdown was established. Karber's method was used to calculate the median lethal dose (LD ) of lead for zebrafish. The50 wild-type and CCM3 gene knockdown zebrafish were exposed to low concentrations of lead (10 mg/L). Development of zebrafish of each group was documented. RESULTS:The LD value for wild-type zebrafish was 31.24 mg/L. Zebrafish50 with CCM3 gene knockdownt were more susceptible to low concentrations of lead-exposure (10 mg/L) than the wild-type by displaying abnormal phenotype,such as decreased body length,pericardial edema,hemorrhage,egg yolk edema, spine distortions. Up to 72 h,after treatment with the low dose,the wild-type zebrafish did not show any abnormal phenotype. CONCLUSION:The CCM3 gene knockdown zebrafish can possibly be used as a sensitive biological monitoring model for cardiovascular toxicants in water.关键词
CCM3基因/铅/斑马鱼/心血管系统/生物监测Key words
CCM3 gene/lead/zebrafish/cardiovascular system/biological monitoring分类
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申碧羚,郭小玲,何云..CCM3基因敲降斑马鱼模型的建立及其在毒理学中的初步应用[J].癌变·畸变·突变,2017,29(3):194-198,204,6.基金项目
国家自然科学基金(81273097,81472998) （81273097,81472998）
中山大学"百人计划"引进人才科研启动基金 （）
