安徽农业大学学报2017，Vol.44Issue(2)：198-202,5.DOI:10.13610/j.cnki.1672-352x.20170419.026
安淮山羊骨骼肌卫星细胞的分离培养与鉴定
Separation and identification of Anhuai goat skeletal muscle satellite cells
摘要
Abstract
The skeletal muscle satellite cells of Anhuai goat were isolated and used for construction of a series of experimental materials.The skeletal muscle satellite cells from longissimus muscle tissue of goats were isolated by collagenase and trypsinase digestion method.The satellite cells were purified by differential adhesion and cultured by passage,cryopreservation and other experimental methods.The marker gene of paired protein box (Pax7) of the cells was detected by PCR and immunocytochemistry technology.After purified by differential adhesion method,the high purity skeletal muscle satellite cells were grown from sphere with a strong refraction to long-shuttle in shape after culturing for a certain time.The results showed that the isolated muscle satellite cells were healthy and had a similar growth style and morphological characteristics.The expression of Pax7 in the cells was detected through immunofluorescent staining and PCR amplication.The analysis result showed that high purity skeletal muscle satellite cells were obtained and these cells were genetic stable.关键词
山羊/骨骼肌卫星细胞/细胞分离/体外培养/免疫荧光染色Key words
goat/skeletal muscle satellite cells/cell isolation/cell culture/immunocytochemistry technology分类
农业科技引用本文复制引用
郑琪,睢梦华,朱龙,吴昊,丁建平,张运海,凌英会..安淮山羊骨骼肌卫星细胞的分离培养与鉴定[J].安徽农业大学学报,2017,44(2):198-202,5.基金项目
安徽省自然科学基金项目(1708085MC61)和安徽省现代农业产业技术体系(2016-2020)共同资助. （1708085MC61）
