安徽农业大学学报2017，Vol.44Issue(2)：203-207,5.DOI:10.13610/j.cnki.1672-352x.20170419.020
部分鸡种Brd2基因SNP和INDEL分析
SNP and INDEL analysis of Brd2 gene in indigenous chicken breeds
摘要
Abstract
Inquiring the SNP and Indel loci of the Brd2 gene belonging to MHC family is of great significance.Capturing high throughput sequencing in Brd2 of 26 Chinese indigenous chicken breeds using Gallus gallus (National Center for Biotechnology Information) as a reference sequence,we screened SNP,Indel and amino acid sites and constructed an evolutionary tree.The results showed that 25 SNP loci and no Indel sites were found in the Brd2 expressed region,while 30 Indel sites and no SNP loci were screened out in the intron region of the 26 chicken breeds.Amino acids had only four missense mutations and the rest were of synonymous mutation.Phylogenetic tree showed that those chicken breeds could be divided into three categories.Anka chicken,Xueshancao chicken,Wenchang chicken,Beijing oil chicken and Xiaoshan chicken were clustered in one class,Taihe chicken,SPF-Leghorn chickens,Game fowl and Guangxi yellow chicken were categorized into another cluster,and the remaining chicken breeds were clustered into the third class that have a relatively low homology.Brd2 polymorphism is conservative but homology is low in indigenous chicken.关键词
鸡/Brd2基因/SNP/Indel/进化树Key words
chickens/Brd2 gene/SNP/Indel/phylogenetic tree分类
农业科技引用本文复制引用
吴允,徐琪,陈国宏,毕榆林,张扬,郭晓敏,李志腾,万方,朱鹏飞,徐璐,常国斌..部分鸡种Brd2基因SNP和INDEL分析[J].安徽农业大学学报,2017,44(2):203-207,5.基金项目
国家自然科学基金项目(31301966)和江苏省六大人才高峰计划共同资助. （31301966）
